Journal of the Korea Institute of Military Science and Technology (한국군사과학기술학회지)

The Korea Institute of Military Science and Technology (한국군사과학기술학회)
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Development of Rapid Antibody-based Therapeutic Platform Correspondence for New Viruses Using Antigen-specific Single Cell Memory B Cell Sorting Technology

항원 특이적 단일 기억 B 세포 분리를 이용한 신종 바이러스 대응 신속 항체 플랫폼 개발

  • Jiyoon Seok (Gene therapy Research Team, Daewoong Pharmaceutical Co, LTD.) ;
  • Suhan Jung (Gene therapy Research Team, Daewoong Pharmaceutical Co, LTD.) ;
  • Ye Gi Han (Gene therapy Research Team, Daewoong Pharmaceutical Co, LTD.) ;
  • Arum Park (Gene therapy Research Team, Daewoong Pharmaceutical Co, LTD.) ;
  • Jung Eun Kim (Chem-Bio Center, Advanced Defense Science & Technology Research Institute, Agency for Defense Development) ;
  • Young Jo Song (Chem-Bio Center, Advanced Defense Science & Technology Research Institute, Agency for Defense Development) ;
  • Chi Ho Yu (Chem-Bio Center, Advanced Defense Science & Technology Research Institute, Agency for Defense Development) ;
  • Hyeongseok Yun (Chem-Bio Center, Advanced Defense Science & Technology Research Institute, Agency for Defense Development) ;
  • Se Hun Gu (Chem-Bio Center, Advanced Defense Science & Technology Research Institute, Agency for Defense Development) ;
  • Seung-Ho Lee (Chem-Bio Center, Advanced Defense Science & Technology Research Institute, Agency for Defense Development) ;
  • Yong Han Lee (Chem-Bio Center, Advanced Defense Science & Technology Research Institute, Agency for Defense Development) ;
  • Gyeunghaeng Hur (Chem-Bio Center, Advanced Defense Science & Technology Research Institute, Agency for Defense Development) ;
  • Woong Choi (Gene therapy Research Team, Daewoong Pharmaceutical Co, LTD.)
  • 석지윤 ((주)대웅제약 유전자신약팀) ;
  • 정수한 ((주)대웅제약 유전자신약팀) ;
  • 한예지 ((주)대웅제약 유전자신약팀) ;
  • 박아름 ((주)대웅제약 유전자신약팀) ;
  • 김정은 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 송영조 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 유치호 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 윤형석 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 구세훈 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 이승호 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 이용한 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 허경행 (국방과학연구소 국방첨단과학기술연구원 Chem-Bio센터) ;
  • 최웅 ((주)대웅제약 유전자신약팀)
  • Received : 2023.10.24
  • Accepted : 2024.01.02
  • Published : 2024.02.05
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Abstract

The COVID-19 pandemic is not over despite the emergency use authorization as can see recent COVID-19 daily confirmed cases. The viruses are not only difficult to diagnose and treat due to random mutations, but also pose threat human being because they have the potential to be exploited as biochemical weapons by genetic manipulation. Therefore, it is inevitable to the rapid antibody-based therapeutic platform to quickly respond to future pandemics by new/re-emerging viruses. Although numerous researches have been conducted for the fast development of antibody-based therapeutics, it is sometimes hard to respond rapidly to new viruses because of complicated expression or purification processes for antibody production. In this study, a novel rapid antibody-based therapeutic platform using single B cell sorting method and mRNA-antibody. High immunogenicity was caused to produce antibodies in vivo through mRNA-antigen inoculation. Subsequently, antigen-specific antibody candidates were selected and obtained using isolation of B cells containing antibody at the single cell level. Using the antibody-based therapeutic platform system in this study, it was confirmed that novel antigen-specific antibodies could be obtained in about 40 days, and suggested that the possibility of rapid response to new variant viruses.

Keywords

Acknowledgement

본 연구는 국방과학연구소의 지원을 받아 수행하였다(UE211112ZD, 311KK5-912888601).

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