대한한의학방제학회지 (Herbal Formula Science)

  • 제32권1호
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  • Pages.99-109
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  • 2024
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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AMPK 활성화를 통한 중금속 유발 산화적 스트레스에 대한 비파엽의 세포 보호 효과

Cytoprotective effect of Eriobotrya japonica L. against the iron-induced oxidative stress through AMPK activation

  • 김민진 (동국대학교 한의과대학 방제학교실) ;
  • 김영은 (동국대학교 한의과대학 방제학교실) ;
  • 박선빈 (동국대학교 한의과대학 방제학교실) ;
  • 배수진 (동국대학교 한의과대학 방제학교실) ;
  • 박광일 (경상대학교 수의과대학 생리학 실험실) ;
  • 박선동 (동국대학교 한의과대학 방제학교실) ;
  • 김영우 (동국대학교 한의과대학 방제학교실)
  • Min-Jin Kim (AI-Bio Convergence DDI Basic Research Laboratory (BRL), School of Korean Medicine, Dongguk University) ;
  • Young-Eun Kim (AI-Bio Convergence DDI Basic Research Laboratory (BRL), School of Korean Medicine, Dongguk University) ;
  • Seon Been Bak (AI-Bio Convergence DDI Basic Research Laboratory (BRL), School of Korean Medicine, Dongguk University) ;
  • Su-Jin Bae (AI-Bio Convergence DDI Basic Research Laboratory (BRL), School of Korean Medicine, Dongguk University) ;
  • Kwang-Il Park (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Sun-Dong Park (AI-Bio Convergence DDI Basic Research Laboratory (BRL), School of Korean Medicine, Dongguk University) ;
  • Young Woo Kim (AI-Bio Convergence DDI Basic Research Laboratory (BRL), School of Korean Medicine, Dongguk University)
  • 투고 : 2024.01.31
  • 심사 : 2024.02.19
  • 발행 : 2024.02.28
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초록

Objectives : In this study, we investigated the cytoprotective effect of Eriobotrya japonica L. (EJ) extract against Arachidonic acid (AA)+iron-induced oxidative stress. Methods : To confirm the cytoprotective effect of EJ against AA+iron-induced oxidative stress in HepG2 cells, it was evaluated by MTT assay, immunoblot anaylsis, and Calcein-AM/propidium iodide (PI) staining. Additionally, the mechanism of action of the cytoprotective effect was evaluated through molecular mechanisms. Results : EJ (100 ㎍/mL) inhibited Arachidonic acid (AA)+iron-induced cell death in a concentration-dependent manner. It also inhibited AA+iron-induced mitochondrial dysfunction and ROS production. EJ activated the LKB1-AMPK signaling pathway. Conclusions : In conclusion, EJ has the ability to protect liver cells from oxidative stress, indicating that it is related to AMPK-LKB1 signaling pathways.

키워드

과제정보

This research was supported by a grant of the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (number: HF20C0212). This work was also supported by the National Research Foundation of Korea (NRF) grant funded by the Korea Government [MSIT] (RS-2023-00218419) (NRF-2022R1I1A3053818) (2022R1A2C1092168).

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