현장농수산연구지 (Journal of Practical Agriculture & Fisheries Research)

국립한국농수산대학교 교육개발센터 (Korea National University of Agriculture and Fisheries)
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고추 소포자를 이용한 반수체 및 배가반수체 생산

Production of Haploid and Doubled Haploid Plants from Isolated Microspore Culture of Hot Pepper (Capsicum annuum L.)

  • 박은준 (LG화학) ;
  • 안율균 (국립한국농수산대학교 원예학부 채소전공) ;
  • 권덕호 (국립한국농수산대학교 원예학부 채소전공) ;
  • 양은영 (국립한국농수산대학교 원예학부 채소전공)
  • Eun Joon Park ( ISC LG Sciencepark, LG) ;
  • Yul Kyun Ahn (Department of Horticulture, Major of Vegetable Crops, Korea National College of Agriculture and Fisheries) ;
  • Doek Ho Kwon (Department of Horticulture, Major of Vegetable Crops, Korea National College of Agriculture and Fisheries) ;
  • Eun Young Yang (Department of Horticulture, Major of Vegetable Crops, Korea National College of Agriculture and Fisheries)
  • 투고 : 2023.12.12
  • 심사 : 2023.12.27
  • 발행 : 2024.01.20
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초록

Haploid/double haploid plants developed from isolated microspores can significantly accelerate plant breeding. Haploid plants can naturally double their chromosomes to create a pure homozygous line of diploid plants. We present a method for producing embryos from isolated microspores of hot peppers (Capsicum annuumL.). We analyzed the polyploidization levels of the regenerated plants. The donor plants produced the optimal stage of microspores following short-term growth under low-intensity light, which resulted in high rates of embryogenesis and cotyledonary embryogenesis. To find an efficient culture method, liquid, doubled-layer, and 2-step cultures were tested. Liquid culture yielded the highest number of embryos, whereas the highest efficiency for cotyledonary embryogenesis was afforded by the doubled-layer culture. When normal cotyledonary embryos were transplanted onto a regeneration medium, they developed into complete plants. From these, 208 plants were tested via flow cytometric analysis, and 35.6% and 72.7% of the chromosomes from the Milyang-jare and LV2319 genotypes, respectively, were found to be spontaneous double haploids. These results are the same as those obtained on analyzing horticultural characteristics, including the size of leaves and the size and shape of fruits. The present study provides information on the practical application of isolated microspore culture of hot peppers, factors that affect embryogenesis, and methods for polyploidy testing.

키워드

과제정보

The work was supported by Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET) through Golden Seed Project, funded by by Ministry of Agriculture, Food and Rural Affairs (MAFRA) (213006-05-1-CGd00) and the Cooperative Research Program for Agriculture Science and Technology Development (PJ012671012018], Rural Development Administration, Republic of Korea.

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