Journal of the Korean Society of Visualization (한국가시화정보학회지)

The Korean Society of Visualization (한국가시화정보학회)
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Size-based Separation of Yeast Cell by Surface Acoustic Wave-induced Acoustic Radiation Force

음향방사력을 이용한 효모세포의 크기별 분리

  • Raihan Hadi Julio (Department of Mechanical Engineering, Chonnam National University) ;
  • Muhammad Soban Khan (Department of Mechanical Engineering, Chonnam National University) ;
  • Mushtaq Ali (Department of Mechanical Engineering, Chonnam National University) ;
  • Ghulam Destgeer (Department of Electrical Engineering, Technical University of Munich) ;
  • Jinsoo Park (Department of Mechanical Engineering, Chonnam National University)
  • Received : 2023.10.10
  • Accepted : 2023.11.28
  • Published : 2023.11.30
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Abstract

The yeast Saccharomyces cerevisiae (S. cerevisiae) is considered an ideal eukaryotic model and has long been recognized for its pivotal role in numerous industrial production processes. Depending on the cell cycle phases, microenvironment, and species, S. cerevisiae varies in shape and has different sizes of each shape such as singlets, doublets, and clusters. Obtaining high-purity populations of uniformly shaped S. cerevisiae cells is crucial in fundamental biological research and industrial operations. In this study, we propose an acoustofluidic method for separating S. cerevisiae cells based on their size using surface acoustic wave (SAW)-induced acoustic radiation force (ARF). The SAW-induced ARF increased with cell diameter, which enabled a successful size-based separation of S. cerevisiae cells using an acoustofluidics device. We anticipate that the proposed acoustofluidics approach for yeast cell separation will provide new opportunities in industrial applications.

Keywords

Acknowledgement

This work was financially supported by ChonnamNational University (Grant number: 2023-1161-01) and the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (No. RS-2023-00210891). The microfluidic devices were fabricated by using a mask aligner (MDA-400S, MIDAS) at Energy Convergence Core Facility in Chonnam National University.

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