Journal of Practical Agriculture & Fisheries Research (현장농수산연구지)

Korea National University of Agriculture and Fisheries (국립한국농수산대학교 교육개발센터)
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Assessment of the Effect of a Disinfectant to Control Vibrio Strain from White Leg Shrimps Cultured in Korea

국내산 양식 흰다리새우 유래의 비브리오균에 대한 소독제의 방제 효과 검증

  • Jin Woo Jun (Department of Aquaculture, Korea National University of Agriculture and Fisheries)
  • 전진우 (국립한국농수산대학교 수산양식전공)
  • Received : 2023.09.18
  • Accepted : 2023.10.13
  • Published : 2023.10.30
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Abstract

White leg shrimps which were cultured in a private aquaculture farm showed abnormal swimming behavior and appetite reduction in July 2023. Then, gradual mortality was observed in the aquaculture farm. During the diagnosis, bacterial strain KNUAFVaSHP08147 was isolated from the hepatopancreas of the dead shrimps. Based on the sequence of 16S rRNA gene, KNUAFVa-SHP08147 was proved to be Vibrio alginolyticus, showing 99.71 % nucleotide identity with that of V. alginolyticus MG54 and GS MYPK1. According to the result of the growth of KNUAFVa-SHP08147, the treatment a commercial disinfectant, VirkonTMS was not proved to be effective to prohibit the growth of Vibrio.

2023년 7월, 국내의 흰다리 새우 양식장에서 양식 중이던 흰다리 새우가 이상유영을 보이며 식욕부진에 시달리며 지속적으로 폐사하였다. 초기에는 일일 평균 폐사율이 0.1%였으나, 3주 후에는 5%까지 증가하였다. 질병 진단 과정 중, 폐사 새우의 간췌장에서 세균(KNUAFVa-SHP08147)이 분리되었다. 본 세균의 16S rRNA 유전자의 시퀀스 분석결과, 분리주 KNUAFVa-SHP08147은 Vibrio alginolyticus로 동정이 되었다. 본 연구에서는, 분리주를 대상으로 비브리오 방제 효과가 있다고 알려져 있는 소독제의 효능을 검증해보았다. 소독제 VirkonTMS의 효능 검증을 위하여, VirkonTMS를 처리한 후 KNUAFVa-SHP08147의 성장 곡선을 조사하였다. 실험 결과, VirkonTMS가 KNUAFVa-SHP08147의 성장에 미치는 영향이 무의미한 것으로 드러나, 소독제 VirkonTMS의 Vibrio 방제 효과는 없는 것으로 드러났다.

Keywords

References

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