Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
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Reduction of Hexavalent Chromium by Shewanella sp. HN-41 in the Presence of Ferric-Citrate

구연산철 환원 조건하에서 Shewanella sp. HN-41에 의한 6가 크롬의 환원

  • Hyemin Park (Department of Agricultural Chemistry, Graduate School, Jeonbuk National University) ;
  • Jin-Hyeob Kwak (Department of Rural Construction Engineering, College of Agriculture & Life Sciences, Jeonbuk National University) ;
  • Ji-Hoon Lee (Department of Agricultural Chemistry, Graduate School, Jeonbuk National University)
  • 박혜민 (전북대학교 대학원 농화학과) ;
  • 곽진협 (전북대학교 농업생명과학대학 지역건설공학과) ;
  • 이지훈 (전북대학교 대학원 농화학과)
  • Received : 2023.08.30
  • Accepted : 2023.09.13
  • Published : 2023.09.30
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Abstract

In the environment, chromium often exists in a highly mobile and toxic form of Cr(VI). Therefore, the reduction of Cr(VI) to less toxic Cr(III) is considered an effective remediation strategy for Cr(VI)-contamination. In this study, the biological reduction of hexavalent chromium was examined at the concentrations of 0.01 mM, 0.1 mM, and 1 mM Cr(VI) by the dissimilatory metal-reducing bacterium, Shewanella sp. HN-41 in the presence of ferric-citrate. With the relatively condensed cell densities, the aqueous phase Cr(VI) was reduced at the proportions of 42%, 23%, and 31%, respectively for the 0.01 mM, 0.1 mM, and 1 mM Cr(VI) incubations, while Fe(III)-citrate was reduced at 95%, 88%, and 73%, respectively. Although the strain HN-41 was not considered to reduce Cr(VI) as the sole electron acceptor for anaerobic metabolism in the preliminary experiment, it has been presumed that outer-membrane c-type cytochromes such as MtrC and OmcA reduced Cr(VI) in the presence of ferric-citrate as the electron acceptor. Since this study indicated the potential of relatively high cell density for Cr(VI) reduction, it might propose a bioremediation strategy for Cr(VI) removal from contaminated waters using engineered systems such as bioreactors employing high cell growths.

Keywords

Acknowledgement

This study was supported by research funds of Jeonbuk National University and in part funded by the Cooperative Research Programs for Agricultural Science and Technology Development (Project No. PJ015716032023 or RS-2021-RD009903), Rural Development Administration, Republic of Korea.

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