Journal of the Korean Applied Science and Technology (한국응용과학기술학회지)

The Korean Society of Applied Science and Technology (한국응용과학기술학회)
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Antioxidant and Antiwrinkle Effects of Persimmon Leaves extract

시엽(Persimmon Leaves) 에탄올 추출물의 항산화와 항주름 효과

  • Sung-Hee Kim (Department of Cosmeceutical Science, Kyungsung University) ;
  • Dong-Hee Kim (Department of industry promotion industrial growth support center) ;
  • Wi-Hye Yeon (Department of Cosmeceutical Science, Kyungsung University) ;
  • Jin-Tae Lee (Department of Cosmeceutical Science, Kyungsung University) ;
  • Young-Ah Jang (Convergence Research Center for Smart Healthcare of KS R&DB Foundation, Kyungsung University)
  • 김성희 (경성대학교 화장품약리학과) ;
  • 김동희 (한국한의약진흥원 산업진흥본부 산업성장지원센터) ;
  • 위혜연 (경성대학교 화장품약리학과) ;
  • 이진태 (경성대학교 화장품약리학과) ;
  • 장영아 (경성대학교 스마트헬스케어융복합연구센터)
  • Received : 2023.05.12
  • Accepted : 2023.06.29
  • Published : 2023.06.30
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Abstract

In this study, we investigated the antioxidant and anti-winkle activity in human fibroblast cell (CCD-986sk) of Persimmon Leaves (PL) as a cosmetic ingredient. As a result of investigating antioxidant activity through electron-donating ability and ABTS+ radical scavenging assay, the PL showed concentration-dependent antioxidant activity similar to ascorbic acid, a control group, at a concentration of 1,000 ㎍/ml. As a result of investigating the anti-wrinkle effect through elastase inhibition and collagenase inhibition assay, the PL showed concentration-dependent antioxidant activity similar to epigallocatechin gallate, a control group, at a concentration of 1,000 ㎍/ml. As a result of measuring the synthesis rate of pro-collagen type I and the inhibition rate of MMP-1 in UVB-induced CCD-986sk cells, the control group EGCG showed a 90.2% pro-collagen synthesis rate at 20 ㎍/ml and PL showed an 88.5% synthesis rate at 30 ㎍/ml. In addition, the inhibition rate of MMP-1 of 33.0% and 40.8% were confirmed in EGCG 20 ㎍/ml and PL 30 ㎍/ml, respectively. As a result of measuring the protein expression of pro-collagen type I and MMP-1 in the PL through western blot, it was confirmed that the protein expression of pro-collagen type I increased, and MMP-1 decreased when the PL was treated together compared to the UVB alone group. According to the above experimental results, it is expected to be used as a natural product material for cosmetics by confirming that the PL prevent photoaging caused by UVB stimulation and have antioxidant and anti-wrinkle effects.

본 연구에서는 시엽의 기능성 화장품 소재로서의 활용 가능성을 평가하기 위해 항산화와 인간섬유아세포인 CCD-986sk 세포에서의 항주름 효과를 알아보았다. 전자공여능 측정, ABTS+ radical 소거능 측정을 통해 항산화 활성을 확인한 결과, 시엽은 1,000 ㎍/ml 농도에서 대조군인 ascorbic acid와 농도 의존적으로 유사한 항산화 활성을 보였다. Elastase 저해 활성 측정, Collagenase 저해 활성 측정을 통해 항주름 효과를 확인한 결과, 시엽은 1,000 ㎍/ml 농도에서 대조군인 epigallocatechin gallate와 농도 의존적으로 유사한 항주름 효과를 나타내었다. UVB로 유도된 CCD-986sk 세포 내 pro-collagen type I의 합성률과 MMP-1의 저해률을 측정한 결과 대조군인 EGCG는 20 ㎍/ml에서 90.2%, 시엽은 30 ㎍/ml에서 88.5%의 pro-collagen 합성률을 보였다. 또한 EGCG 20 ㎍/ml와 시엽 30 ㎍/ml에서 각각 33.0%, 40.8%의 MMP-1 저해율을 확인하였다. Western blot을 통하여 시엽의 pro-collagen type I과 MMP-1의 단백질 발현량을 측정한 결과 UVB 단독군 대비 시엽을 함께 처리했을 때 pro-collagen type I의 단백질 발현량이 증가하고 MMP-1의 단백질 발현량이 감소하는 것을 확인하였다. 위 실험결과에 따라 시엽이 UVB 자극에 의한 광노화를 예방하고 항산화, 항주름 효과가 있음을 확인하여 화장품의 천연물 소재로서의 이용이 기대된다.

Keywords

References

  1. J. H. Jeong, "한국인의 피부노화 특징 및 발생기전", Journal of the society of cosmetic scientists of Korea, Vol.27, No.2, pp. 31-35, (2001). 
  2. J. M. Kim, S. W. Jeon, W. G. Lee, H. J. Nam, Y. B. Ki, "Study of Preventing Methods for Skin Aging and Wrinkles", Korean Journal of Oriental Physiology & Pathology, Vol.24, No.4, pp. 533-542, (2010). 
  3. Uitto, Jouni, "Understanding Premature Skin Aging", The New England journal of medicine, Vol.337, No.20, pp. 1463-1465, (1997).  https://doi.org/10.1056/NEJM199711133372011
  4. Alexandra Amaro-Ortiz, Betty Yan, John A. D'Orazio1, "Ultraviolet Radiation, Aging and the Skin: Prevention of Damage by Topical cAMP Manipulation", Molecules, Vol.19, No.5, pp. 6202-6219, (2014).  https://doi.org/10.3390/molecules19056202
  5. Esra Birben, Umit Murat Sahiner, Cansin Sackesen, Serpil Erzurum, Omer Kalayci, "Oxidative stress and antioxidant defense", World Allergy Organization Journal, Vol.5, No.1, pp. 9-19, (2012).  https://doi.org/10.1097/WOX.0b013e3182439613
  6. Rajindar S. Sohal, Richard Weindruch, "Oxidative Stress, Caloric Restriction, and Aging", Science, Vol.273, No.5271, pp. 59-63, (1996).  https://doi.org/10.1126/science.273.5271.59
  7. S. J. Seo1, G. E. Park, M. J. Jang, Y. S. Lee, "Anti-aging Effects of Ailanthi Radicis Cortex Extract", Journal of Investigative Cosmetology, Vol.14, No.2, pp. 179-186, (2018).  https://doi.org/10.15810/jic.2018.14.2.005
  8. Yahong Wang, Adam R. Johnson, Qi-Zhuang Ye, Richard D. Dyer, "Catalytic Activities and Substrate Specificity of the Human Membrane Type 4 Matrix Metalloproteinase Catalytic Domain", Journal of Biological Chemistry, Vol.274, No.46, pp. 33043-33049, (1999).  https://doi.org/10.1074/jbc.274.46.33043
  9. Helena Laronha, Jorge Caldeira, "Structure and Function of Human Matrix Metalloproteinases", Cells, Vol.9, No.5, pp. 1076, (2020). 
  10. E. Y. Choi, H. Y. Sohn, J. T. Lee, "The Antioxidant and Anti-aging Effects of Treatment with Schisandra chinensis Seed Fractions in UVB-irradiated Human HaCaT cells", Journal of Life Science, Vol.29, No.10, pp. 1071-1079, (2019).  https://doi.org/10.5352/JLS.2019.29.10.1071
  11. C. S. Kwak, J. W. Yang, "Prevention Effect of Prunus Persica Flos Extract from Reactive Oxygen Species Generation and Matrix Metalloproteinase Production Induced by UVB Irradiation in Human Skin cells", Asian Journal of Beauty Cosmetol, Vol.14, No.2, pp. 179-190, (2016).  https://doi.org/10.20402/ajbc.2016.0043
  12. J. E. Kim, Y. S. Choi, H. K. Kim, Y. A. Jang, "Collagen synthesis ability and inhibitory effect of MMPs in Keratinocytes of Lysimachia christinae Hance Extract", Journal of the Korean Applied Science and Technology, Vol.37, No.4, pp. 820-829, (2020).  https://doi.org/10.12925/JKOCS.2020.37.4.820
  13. Jie Ma, Xiao-yu Liu, Kyung-Hee Noh, Myo-Jeong Kim, and Young-Sun Song, "Protective Effects of Persimmon Leaf and Fruit Extracts against Acute Ethanol-Induced Hepatotoxicity", Journal of Food Science and Nutrition, Vol.12, pp. 202-208, (2007).  https://doi.org/10.3746/jfn.2007.12.4.202
  14. K. H. Yoo, J. M. Jeong, "Antioxidative and Antiallergic Effect of Persimmon Leaf Extracts", Journal of Food Science and Nutrition, Vol.38, No.12, pp. 1691-1698, (2009).  https://doi.org/10.3746/jkfn.2009.38.12.1691
  15. Jie Yu, Yanyan Song, Bing Yu, Jun He, Ping Zheng, Xiangbing Mao, Zhiqing Huang, Yuheng Luo, Junqiu Luo, Hui Yan, Quyuan Wang, Huifen Wang, Daiwen Chen, "Tannic acid prevents post-weaning diarrhea by improving intestinal barrier integrity and function in weaned piglets", Journal of Animal Science and Biotechonology, Vol.11, No.87, (2020). 
  16. Blois M. S, "Antioxidant Determinations by the Use of a Stable Free Radical" Nature, Vol.181, No.4617, pp. 1199-1200, (1958).  https://doi.org/10.1038/1811199a0
  17. R Re, N Pellegrini, A Proteggente, A Pannala, M Yang, C Rice-Evans, "Antioxidant activity applying an improved ABTS radical cation decolorization assay", Free Radical Biology & Medicine, Vol.26, No.9/10, pp. 1231-1237, (1999).  https://doi.org/10.1016/S0891-5849(98)00315-3
  18. Richard J. P. Cannell, Stephen J. Kellam, Ania M. Owsianka, John M. Walker, "Results of a Large Scale Screen of Microalgae for the Production of Protease Inhibitors", Planta Med, Vol.54, No.1, pp. 10-14, (1988).  https://doi.org/10.1055/s-2006-962319
  19. J. Carmichael, W. G. Degraff, A. F. Gazdar, J. D. Minna, J. B. Mitchell, "Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing", Cancer Res, Vol.47, No.4, pp. 936-942, (1987). 
  20. M. S. Seo1, Y. A. Jang, J. T. Lee, "The Study of Cosmeceutical Activities from Lentinula edodes extracts and Application a Natural Cosmetic Material", Journal of Oil & Applied Science, Vol.35, No.4, pp. 1003-1012, (2018). 
  21. Y. A. Jang, J. T. Lee, "Effect of Unripe Apple Peel Water Extracts on Tyrosinase Activity and Melanin Production in B16F10 Melanoma Cells", Journal of Life Science, Vol.28, No.8. pp. 900-907, (2018).  https://doi.org/10.5352/JLS.2018.28.8.900
  22. Y. A. Jang, S. H. Park, B. A. Kim, J. Y. Park, Y. O. Jeoung, J. T. Lee, "Effect of ethanol extract of Lotus Rhizome and node of Lotus Rhizome", Journal of Oil & Applied Science, Vol.34, No.3. pp. 657-665, (2017). 
  23. Ronald L Prior, Xianli Wu, Karen Schaich, "Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements", Journal of agricultural and food chemistry, Vol.53, No.10, pp. 4290-4302, (2005).  https://doi.org/10.1021/jf0502698
  24. Y. H. Kim, Y. J. Lee, S. O. Park, S. J. Lee, O. H. Lee, "Antioxidant Compounds and Antioxidant Activities of Fermented Black Rice and Its Fractions", Korean Journal of Food Science and Technology, Vol.45, No.2, pp. 262-266, (2013).  https://doi.org/10.9721/KJFST.2013.45.2.262
  25. M. J. Jang, D. H. Jun, S. H. Kim, S. I. Han, J. T. Lee, "Anti-wrinkle Effect of Chestnut Leaf", Journal of Life Science, Vol.23, No.6, pp. 751-756, (2013).  https://doi.org/10.5352/JLS.2013.23.6.751
  26. D. H. Kim, T. S. Park, J. H. Son, "Anti-wrinkle Activities Verification of Buplerum falcatum Extracts on CCD-986sk", Journal of Applied Biological Chemistry, Vol.58, No.2, pp. 183-187, (2015).  https://doi.org/10.3839/jabc.2015.029
  27. Hans Christian Wulf, Jane Sandby-Moller, Takasi Kobayasi. Robert Gniadecki, "Skin aging and natural photoprotection", Micron, Vol.35, pp. 185-191, (2004).  https://doi.org/10.1016/j.micron.2003.11.005
  28. K. E. Lee, S. H. Son, S. G. Kang, "The Coffee Sliver Skin Extracts from Coffee Beans Exhibited Cosmetic Properties with Antioxiant Activity and Inhibitory Effects for Elastase, Collagenase and Tyrosinase", Journal of the Society of Cosmetic Scientists of Korea, Vol.44, No.1, pp. 39-48, (2018).  https://doi.org/10.15230/SCSK.2018.44.1.39
  29. Jason Yang, S. Nandi, "Growth of Cultured Cells Using Collagen as Substrate", International Review of Cytology, Vol.91, pp. 249-286, (1983).  https://doi.org/10.1016/S0074-7696(08)62340-2
  30. J. W. Kim, J. M. Ahn, O. J. Kwon, S. H. Kim, "β-Glucan Contents and Anti-wrinkling Effects of Brown Rice Phellinus linteus Mycelium Extracts Fermented with Lactobacillus plantarum", Asian Journal of Beauty and Cosmetology, Vol.14, No.2, pp. 127-137, (2016).  https://doi.org/10.20402/ajbc.2016.0020
  31. S. Y. Lee, J. Y. Lee, "Inhibitory Efficacy of Smilax china L. on Pro-collagen Type-1 Activity and MMP-1 Gene Expression in Fibroblasts", Journal of Life Science, Vol.23, No.10, pp. 1239-1245, (2013). https://doi.org/10.5352/JLS.2013.23.10.1239