Journal of Periodontal and Implant Science

  • 제53권4호
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  • Pages.259-268
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  • 2023
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  • 2093-2278(pISSN)
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  • 2093-2286(eISSN)
대한치주과학회 (Korean Academy of Periodontology)
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The optimal dosage of hyaluronic acid for bone regeneration in rat calvarial defects

  • Ling Li (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Jungwon Lee (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Young-Dan Cho (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Sungtae Kim (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Yang-Jo Seol (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Yong-Moo Lee (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University) ;
  • Ki-Tae Koo (Department of Periodontology and Dental Research Institute, School of Dentistry, Seoul National University)
  • 투고 : 2022.06.20
  • 심사 : 2022.10.19
  • 발행 : 2023.08.31
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초록

Purpose: Hyaluronic acid (HA) affects angiogenesis and promotes the migration and differentiation of mesenchymal cells, thereby activating the osteogenic ability of osteoblasts. Although studies on the action of HA during bone regeneration are being actively conducted, the optimal dose of HA required for bone regeneration remains unclear. Therefore, the purpose of this study was to elucidate the most effective HA dose for bone formation using a rat critical-size defect model. Methods: Thirty rats were randomly divided into 5 groups, with 6 rats in each group. An absorbable collagen sponge soaked with HA or saline was used to fill an 8-mm defect, which was then covered with a collagen membrane. Different treatments were performed for each group as follows: (1) saline control, (2) 1 mg/mL HA, (3) 25 mg/mL HA, (4) 50 mg/mL HA, or (5) 75 mg/mL HA. After a healing period of 4 weeks, micro-computed tomography and histological analysis were performed. The obtained values were analyzed using analysis of variance and the Tukey test (P<0.05). Results: At week 4, the 75 mg/mL HA group had the highest bone volume/total volume ratio, new bone, and bone fill among the 5 groups, and these values were significantly different from those observed in the control group (P<0.01) and 1 mg/mL HA group (P<0.001). More active bone formation was observed in the higher-dose HA groups (25 mg/mL, 50 mg/mL, and 75 mg/mL HA), which included a large amount of woven bone. Conclusions: The 75 mg/mL HA group showed better bone formation than the other groups (1, 25, and 50 mg/mL HA and control).

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과제정보

The project was supported by a grant from the GENOSS (NCR19008), Republic of Korea.

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