Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Comparative Evaluation of Biological Activities and Active Compounds of Some Invasive Alien Plants

주요 생태계 교란 외래식물의 생리활성 비교 평가 및 유효성분

  • So Jin Kim (Faculty of Biotechnology, Jeju National University) ;
  • Min Gun Kim (Faculty of Biotechnology, Jeju National University) ;
  • Kyung-Hwan Boo (Faculty of Biotechnology, Jeju National University) ;
  • Chang Sook Kim (Faculty of Biotechnology, Jeju National University)
  • 김소진 (제주대학교 생명공학부) ;
  • 김민건 (제주대학교 생명공학부) ;
  • 부경환 (제주대학교 생명공학부) ;
  • 김창숙 (제주대학교 생명공학부)
  • Received : 2023.03.15
  • Accepted : 2023.04.19
  • Published : 2023.08.01
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Abstract

To evaluate functional biomaterials of 5 invasive alien plants, total polyphenol and flavonoid contents, antioxidant activity, anti-inflammatory activity, and antibacterial effect were measured. The total polyphenol and flavonoid contents of the extracts were in the order of Rumex acetosella L. > Hypochaeris radicata L. ≥ Lactuca scariola L. > Humulus japonicus Siebold & Zucc. ≥ Solanum viarum Dunal. The DPPH and ABTS radical scavenging activities of the extract were the highest in R. acetosella and correlated well with the total polyphenol contents. In RAW 264.7 cells stimulated with lipopolysaccharide (LPS), nitric oxide (NO) and prostaglandin E2 (PGE2) production inhibitory effect of the extracts (100 ㎍ SE/mL) were 20~60% and 10~70%, respectively, showing the highest inhibitory effect in R. acetocella. The extracts of R. acetosella, H. japonicus and S. viarum showed antibacterial activity against food poisoning-causing microorganisms such as Bacillus subtilis, Escherichia coli, Vibrio parahaemolyticus and Vibrio vulnificus. Furthermore, the H. japonicus extract was found to have effective antibacterial activity against oral microorganisms such as Enterococcus faecalis, Lacticaseibacillus casei, Rothia dentocariosa, Staphylococcus epidermidis and Streptococcus mutans, and its major active ingredients were predicted to be pentadecylic acid, palmitic acid and clionasterol. These results suggest that alien plants have potential as biomaterials with antioxidant, anti-inflammation and antibacterial effects.

본 연구는 제주지역에서 대량 발생하고 있는 생태계 교란 외래식물을 기능성 바이오소재로서의 이용 가능성을 확인하기 위한 연구의 일환으로서, 5종 외래식물체의 70% 에탄올 추출물을 대상으로 항산화, 항염증 및 항균 효과와 유효성분을 조사하였다. 외래식물의 총 폴리페놀 및 플라보노이드 함량은 애기수영 > 서양금혼초 ≥ 가시상추 > 환삼덩굴 ≥ 왕도깨비가지 순서로 나타났다. 외래식물의 DPPH 및 ABTS 라디칼 소거능 역시 애기수영에서 가장 높았으며, 항산화 효과는 식물 추출물의 총 폴리페놀 및 플라보노이드 함량과 연관된 것으로 확인되었다. LPS로 자극시킨 RAW 264.7 세포에서 외래식물체 에탄올 추출물(100 ㎍ SE/mL)의 NO 및 PGE2 생성 억제 효과는 각각 약 20~60%와 10~70%로 나타났고, 애기수영 및 환삼덩굴이 다른 3종에 비해 우수한 것으로 나타났다. 4종의 식중독 유발 미생물 및 5종의 구강 미생물을 대상으로 식물 추출물의 항균 효과를 조사한 결과, 환삼덩굴, 애기수영 및 왕도깨비가지의 에탄올 추출물은 B. subtilis, E. coli, V. parahaemolyticus 및 V. vulnificus 4종 식중독균 모두에 대해 강한 항균 활성을 보였다. 구강충치 유발 미생물인 E. faecalis, L. casei, R. dentocariosa, S. epidermidis, S. mutans에 대한 항균 효과는 환삼덩굴 추출물이 효과적이었으며, 항균 활성에 관여하는 주 유효성분은 pentadecylic acid, palmitic acid, clionasterol 등인 것으로 예측되었다. 이상의 결과는 생태계 교란 외래식물, 특히 애기수영 및 환삼덩굴의 기능성을 갖는 유효성분 분리 및 이용화 연구에 중요한 기초자료가 될 것이며, 항산화, 항염증 및 항균제 개발을 위한 바이오소재 발굴 등에도 중요한 정보를 제공할 수 있을 것으로 사료된다.

Keywords

Acknowledgement

본 연구는 2022년도 환경부지정 제주녹색환경지원센터(과제번호 3-70-76-13-4)의 연구비 지원에 의해 이루어진 것으로 감사드립니다.

References

  1. Anagnostopoulou, M.A., P. Kefalas, V.P. Papageorgiou, A.N. Assimopoulou and D. Boskou. 2006. Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis). Food Chem. 94(1):19-25. https://doi.org/10.1016/j.foodchem.2004.09.047
  2. Bauer, A.W., M.M. Kibby, J.C. Sherris and M. Turck. 1966. Anti-biotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45(4):493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
  3. Blois, M.S. 1958. Antioxidant determination by the use of a stable free radical. Nature 181:1199-1200. https://doi.org/10.1038/1811199a0
  4. Bohlmann, F. and R. Bohlmann. 1980. Three guaianolides from Hypochoeris radicata. Phytochemistry 19:2045-2046. https://doi.org/10.1016/0031-9422(80)83041-X
  5. Choi, B.S., D.Y. Song, C.G. Kim, B.H. Song, S.H. Woo and C.W. Lee. 2010a. Allelopathic effects of common ragweed (Ambrosia artemisifolia var. elation) on the germination and seedling growth of crops and weeds. Kor. J. Weed Sci. 30(1):34-42 (in Korean). https://doi.org/10.5660/KJWS.2010.30.1.034
  6. Choi, I.Y., Y.J. Song and W.H. Lee. 2010b. DPPH radical scavenging effect and antimicrobial activities of some herbal extracts. Kor. J. Hort. Sci. Technol. 28:871-876 (in Korean).
  7. Choi, Y.J., J.H. Jeong, J.H. Jeong, K.R. Kim, Y.S. Lee and J.W. Oh. 2020. Revision of threshold levels for evoking pollinosis to oak, pine, Japanese hop, and ragweed in the metropolitan area Seoul, Korea. Allergy Asthma Respir Dis. 8(4)199-205. https://doi.org/10.4168/aard.2020.8.4.199
  8. Folin, O. and W. Denis. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12:239-249. https://doi.org/10.1016/S0021-9258(18)88697-5
  9. Hertog, M.G.L., P.C.H. Hollman and D.P. Venema. 1992. Optimization of a quantitaive HPLC determination of potentially anticarcinogenic flavonoids in vegetables and fruits. J. Agric. Food Chem. 40(9):1591-1598. https://doi.org/10.1021/jf00021a023
  10. Hur, J.Y., C.H. Jeong and K.H. Shim. 2003. Chemical components of Humulus japonicus leaves and stalks. J. Agriculture & Life Sciences 37(1):1-7.
  11. Hwang, H.J., J.S. Yu, H.Y. Lee, D.J. Kwon, W. Han, S.I. Heo and S.Y. Kim. 2014. Evaluation on deodorization effect and anti-oral microbial activity of essential oil from Pinus koraiensis. Korean J. Plant Res. 27:1-10 (in Korean). https://doi.org/10.7732/kjpr.2014.27.1.001
  12. Kang, S.E., K.B. Park, Y.J. Jang, G.S. Lee and C.D. Son. 2022. Richardia brasiliensis Gomes (Rubiaceae), a new invasive alien plant in Korea. Korean J. Plant Res. 35(2):242-247 (in Korean).
  13. Kang, Y.H., Y.K. Park and G.D. Lee. 1996. The nitrite scavenging and electron donating ability of phenolic compounds. Korean J. Food Sci. Technol. 28(2):232-239 (in Korean).
  14. Katsube, T., H. Tabata, Y. Ohta, Y. Yamasaki, E. Anuurad, K. Shiwaku. 2004. Screening for antioxidant activity in edible plant products: Comparison of low-density lipoprotein oxidation assay, DPPH radical scavenging assay, and FoilinCiocalteu assay. J. Agric. Food Chem. 52:2391-2396. https://doi.org/10.1021/jf035372g
  15. Kim, H.H., K. Mizuno, D.B. Kim, H.S. Lee and W.S. Kong. 2020. Distribution of invasive alien plants on the islands of the Korean Peninsula based on flora data. Korean J. Environ. Biol. 38(3):392-403 (in Korean). https://doi.org/10.11626/KJEB.2020.38.3.392
  16. Kim, S.J., I.B. Park, S.G. Kang, D.O. Chung and S.T. Jung. 2005. Anticariogenic activity and glucosyltransferase inhibition of phenolic compounds. Korean J. Food Culture 20:603-607 (in Korean).
  17. Kim, S.J., J.Y. Shin, Y.M. Park, K.M. Chung, J.H. Lee and D.H. Kweon. 2006. Investigation of antimicrobial activity and stability of ethanol extracts of licorice root (Glycyrrhiza glabra). Korean J. Food Sci. Technol. 38:241-248 (in Korean).
  18. Kim, S.Y., E.J. Jang, E.J. Kim, J.H. Jeong, S.E. Kang, Y.H. Gil and C.D. Son. 2022. Vulpia octoflora (Walter) Rydb. (Poaceae), a new invasive alien plant in Korea. Korean J. Plant Res. 35(5):607-613 (in Korean).
  19. Kim, T.K., H.C. Kim, J.Y. Song, Y.S. Ha, J.H. Kang, S.B. Woo and C.K. Song. 2011. Herbicidal activity of aqueous extracts from Solanum viarum (Dunal). Korean J. Organic Agriculture 19:65-82 (in Korean).
  20. Kim, Y.H., J.H. Kil, S.M. Hwang and C.W. Lee. 2013. Spreading and distribution of Lactuca scariola, invasive alien plant, by habitat types in Korea. Weed Turf. Sci. 2(2):138-151 (in Korean). https://doi.org/10.5660/WTS.2013.2.2.138
  21. KLIC (Korean Law Information Center). 2021. Biodiversity conservation and utilization act. http://www.law.go.kr (Accessed May 4, 2021).
  22. Ko, H.M., T.K. Eom, S.K.Song, G.Y. Jo and J.S. Kim. 2017. Tyrosinase and α-glucosidase inhibitory activities and antioxidant effects of extracts from different parts of Hypochaeris radicata. Korean J. Medicinal Crop Sci. 25(3):139-145 (in Korean). https://doi.org/10.7783/KJMCS.2017.25.3.139
  23. Lazarov, S., M. Balutsov and E. Ianev. 2000. The role of bacterial endotoxins, receptors and cytokines in the pathogenesis of septic (endotoxin) shock. Vutr. Boles. 32:33-40.
  24. Lee, I.Y., S.H. Kim and S.H. Hong. 2021. Occurrence characteristic and management of invasive weeds, Ambrosia artemisiifolia, Ambrosia trifida and Humulus japonicus. Weed Turf. Sci. 10(3):227-242 (in Korean).
  25. Lee, K.S., M.G. Kim and K.Y. Lee. 2004. Antimicrobial effect of the extracts of cactus chounnyouncho (Opuntia humifusa) against food borne pathogens. J. Korean Soc. Food Sci. Nutr. 33:1268-1272 (in Korean). https://doi.org/10.3746/jkfn.2004.33.8.1268
  26. Lee, S.H. and M.J. Kim. 2019. Antimicrobial effect of natural plant extracts against periodontopathic bacteria. J. Korea Contents Association 19:242-255 (in Korean).
  27. Lee, S.J., H.J. Bu, J.A. Lee and D.S. Jung. 2005. Screening of plants in Jeju for whitening materials in cosmeceutical. J. Sco. Cosmet. Scientists Korea 31(1):115-119 (in Korean).
  28. Maruta, Y., Y. Fukushi, K. Ohkawa, Y. Nakanishi, S. Tahara and J. Mizutani. 1995. Antimicrobial stress compounds from Hypochoeris radicata. Phytochemistry 38:1169-1173. https://doi.org/10.1016/0031-9422(94)00792-R
  29. Moreno, M.I.N., M.I. Isla, A.R. Sampietro, W.A. Vattuone. 2000. Comparison of the free radical-scavenging activity of propolis from several regions of argentina. J. Ethnopharmacol. 71:109-114. https://doi.org/10.1016/S0378-8741(99)00189-0
  30. Pandey, K.B. and S.I. Rizvi. 2009. Plant polyphenols as dietary antioxidants in human health and disease. Oxid. Med. Cell Longev. 2:270-278. https://doi.org/10.4161/oxim.2.5.9498
  31. Park, Y.O. and H.S. Lim. 2010. Antimicrobial activity of bamboo (Sasa borealis) leaves fraction extracts against food poisoning bacteria. J. Korean Soc. Food Sci. Nutr. 39:1745-1752 (in Korean). https://doi.org/10.3746/jkfn.2010.39.12.1745
  32. Re, R., N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, C. Rice-Evans. 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic. Biol. Med. 26:1231-1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  33. Rosan, B. and R.J. Lamont. 2000. Dental plaque formation. Microbes Infect. 2:1599-1607. https://doi.org/10.1016/S1286-4579(00)01316-2
  34. Ryu, Y.K. 2017. Effect of Humulus japonicus in models of Parkinson's disease. Department of Toxicology Evaluation, MS Thesis, Konyang Univ., Daejeon, Korea. p. 58.
  35. Sim, S., J.S. Kim, D.P. Jin, W. Lee, C.W. Hyun and J.H. Kim. 2022. New record for alien plant, Urtica dioica L. (Urticaceae) in Korea. Korean J. Plant Res. 35(1):100-108 (in Korean).
  36. Storck, M., M Schilling, K. Burkhardt, R. Prestel, D. Abendroth and C. Hammer. 1994. Production of proinflammatory cytokines and adhesion molecules in ex-vivo xenogenetic kidney perfusion. Transpl. Int. 7:647-649. https://doi.org/10.1111/j.1432-2277.1994.tb01464.x
  37. Yang, E.J., H.H. Kwang, H.R. Choi, S.Y. Kim and C.C. Hyun. 2018. Anti-inflammatory activity of rumex acetosella extracts from Jeju island. KSBB Journal 33:155-160. https://doi.org/10.7841/ksbbj.2018.33.3.155
  38. Yang, K.M., S.M. Song, D. Lee, W.J. Yoon, C.H, Kim and C.S. Kim. 2019. Anti-inflammatory and anti-atopic effects of crude extracts and solvent fractions of Phormium tenax leaf. Korean J. Plant Res. 32:433-441 (in Korean).
  39. Yang, S.H., S.J. Park, R.U. Lee, J.W. Lee, J.H. Lee, J.H. Cha, S.J. Jeong. 2016. Antibacterial effects on oral microorganism and inhibitory effects of halitosis of Omija (Schizandra chinensis baillon) extract. J. Korean Oral Health Sci. 4:93-98 (in Korean).
  40. Yang, Y.H. and M.H. Kim. 2002. A study on the distribution and vegetation of Hypochoeris radicata community group in Jeju island. Korean J. Plant Res. 15:227-236 (in Korean).