Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Temperature Effect on the Growth and Odorous Material (2-MIB) Production of Pseudanabaena redekei

온도가 남조류 Pseudanabaena redekei의 성장과 냄새물질(2-MIB) 생산에 미치는 영향

  • Jaehyun Kim (Department of Environment and Health Science, Konkuk University) ;
  • Keonhee Kim (Human and Eco Care Center, Konkuk University) ;
  • Chaehong Park (Human and Eco Care Center, Konkuk University) ;
  • Hyunjin Kim (Pyunghwa Engineering Consultants) ;
  • Soon-Jin Hwang (Department of Environment and Health Science, Konkuk University)
  • 김재현 (건국대학교 상허생명과학대학 환경보건과학과) ;
  • 김건희 (건국대학교 휴먼앤에코케어센터) ;
  • 박채홍 (건국대학교 휴먼앤에코케어센터) ;
  • 김현진 (주식회사 평화엔지니어링) ;
  • 황순진 (건국대학교 상허생명과학대학 환경보건과학과)
  • Received : 2023.06.11
  • Accepted : 2023.06.27
  • Published : 2023.06.30
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Abstract

Cyanobacteria Pseudanabaena strains are known to produce 2-MIB(odorous material) in freshwater systems, thereby causing problems in water use. However, their physiological responses to environmental factors in relation with 2-MIB production is not well explored. This study was conducted to evaluate the effect of temperature on the growth and 2-MIB production of Pseudanabaena redekei. The experimental cyanobacteria strains were separated from the Uiam Reservoir (North Han River) and cultured in the BG-11 medium. Temperature was set to 10, 15, 20, 25, and 30℃ for the experiment, in the reflection of the seasonal water temperature variation in situ. For each temperature treatment, cyanobacterial biomass(Chl-a) and 2-MIB concentration (intra-cellular and extra-cellular fractions) were measured every 2 days for 18 days. Both maximal growth and total 2-MIB production of P. redekei appeared at 30℃. While intra-cellular 2-MIB contents were similar (26~29 ng L-1) regardless of treated temperatures, extra-cellular 2-MIB concentration was higher only in high temperature conditions (25~30℃), indicating that the extents of 2-MIB biosynthesis and release by P. redekei vary with temperature. The 2-MIB productivity of P. redekei was much higher in low-temperature conditions (10~15℃) than high temperature conditions (25~30℃). This study demonstrated that temperature was a critical factor contributing to 2-MIB biosynthesis and its release in cell growth (r=0.605, p<0.01). These results are important to understand the dynamics of 2-MIB in the field and thereby provide basic information for managing odorous material in drinking water resources.

담수생태계에서 Pseudanabaena는 이취미 물질(2-MIB)을 생산하여 물이용을 저해하는 남조류로 알려져 있으나, 2-MIB 생산과 관련하여 이 속에 속한 균주들의 동태에 미치는 환경요인에 대한 연구는 미흡한 실정이다. 본 연구에서는 P. redekei의 온도에 따른 성장과 2-MIB 생산 특성을 평가하였다. 실험에 사용된 남조류는 북한강 수계의 의암댐에서 분리하여 BG-11 배지에서 배양하였다. 실험온도 조건은 현장의 계절별 온도를 반영하여 10, 15, 20, 25, 30℃로 설정하여 18일간 배양하였으며, 2일 간격으로 전체 생물량(Chl-a)과 세포 내 함량과 세포 외 농도로 구분하여 2-MIB 농도를 측정하였다. 최고 생물량(Chl-a)과 가장 높은 총 2-MIB 농도는 각각 30℃, 25℃에서 나타났다. 세포 내 2-MIB 함량은 처리온도에 관계없이 비슷한 농도(26~29 ng L-1)를 나타냈지만, 세포 외 2-MIB 농도는 25~30℃의 고온 조건에서만 높게 나타나 온도에 따라 2-MIB 합성과 세포외 배출 특성이 다른 경향을 보여주었다. 2-MIB 생산력은 고온 조건에 비해 10~15℃의 저온 조건에서 더 높았으며, 25℃ 이상의 온도에서는 매우 낮았다. 본 연구 결과는 수온이 P. redekei의 성장과 연계하여 세포 내 2-MIB 합성과 세포 외 용출에 관여하는 중요한 인자임을 증명하였으며, 이를 통해 상수원에서 이취미 물질 발생 원인종의 성장과 이취미 물질의 동태를 이해하고, 동시에 이취미 물질 관리에 대한 중요한 기초 정보를 제공하고자 하였다.

Keywords

Acknowledgement

본 연구는 한국환경산업기술원의 '수생태계 건강성 확보 기술개발사업'인 '최첨단 위치기반 USBL ROV로봇을 탑재한 휴면포자 함유 퇴적물 준설 기술 개발' 과제에 의해 수행되었습니다(2022003040003).

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