Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Hepatoprotective effect of Samchulgeonbi-tang via Nrf2 Activation

Nrf2활성화를 통한 삼출건비탕(蔘朮健脾湯)의 간세포보호효과

  • Ye Lim Kim (College of Korean Medicine, Daegu Haany University) ;
  • Hyo Jeong Jin (College of Korean Medicine, Daegu Haany University) ;
  • Sang Mi Park (College of Korean Medicine, Daegu Haany University) ;
  • Sung Hui Byun (College of Korean Medicine, Daegu Haany University) ;
  • Chang Hyun Song (College of Korean Medicine, Daegu Haany University) ;
  • Sang Chan Kim (College of Korean Medicine, Daegu Haany University)
  • 김예림 (대구한의대학교 한의과대학) ;
  • 진효정 (대구한의대학교 한의과대학) ;
  • 박상미 (대구한의대학교 한의과대학) ;
  • 변성희 (대구한의대학교 한의과대학) ;
  • 송창현 (대구한의대학교 한의과대학) ;
  • 김상찬 (대구한의대학교 한의과대학)
  • Received : 2023.05.12
  • Accepted : 2023.05.22
  • Published : 2023.05.31
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Abstract

Objectives : Oxidative stress is an important cause of many diseases including liver injury. Therefore, adequate regulation of oxidative stress plays a pivotal role in maintaining liver function. Until recently, there has been no studies on the hepatoprotective effect of Samchulgeonbi-tang (SCGBT). Therefore, the hepatoprotective effect of SCGBT was investigated in HepG2 cells. In this study, oxidative stress was induced by arachidonic acid (AA) and iron. Methods : To analyze the hepatoprotective effects of SCGBT against oxidative stress induced by AA + iron, the cell viability, apoptosis-related proteins and intracellular ROS, glutathione (GSH), and mitochondrial membrane permeability (MMP) were measured. In addition, nuclear factor erythroid 2-related factor 2 (Nrf2) transcription activation and expressions of Nrf2 target gene were analyzed through immunoblot analysis. Results : SCGBT increased the cell viability from AA + iron - induced cell death and inhibited apoptosis by regulating apoptosis related proteins. SCGBT protected cells by inhibiting ROS production, GSH depletion, and MMP degradation against AA + iron induced oxidative stress. Furthermore, Nrf2 activation was increased by SCGBT, and the Nrf2 target genes were also activated by SCGBT. Conclusions : These results suggest that the SCGBT has a hepatocyte protection effect and antioxidant effect from AA + iron induced oxidative stress.

Keywords

Acknowledgement

This study was supported by the National Research Foundation of Korea funded by Korea government (MSIP) (Grant No.2018R1A5A2025272)

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