Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Anti-inflammatory effect of Sihosogan-tang via inhibition of NF-κB and MAPK cascade

NF-κB와 MAPK억제를 통한 시호소간탕(柴胡疏肝湯)의 항염증효과

  • Hyo Jeong Jin (College of Korean Medicine, Daegu Haany University) ;
  • Sang Mi Park (College of Korean Medicine, Daegu Haany University) ;
  • Ye Lim Kim (College of Korean Medicine, Daegu Haany University) ;
  • Sung Hui Byun (College of Korean Medicine, Daegu Haany University) ;
  • Sang Chan Kim (College of Korean Medicine, Daegu Haany University)
  • 진효정 (대구한의대학교 한의과대학 방제학교실) ;
  • 박상미 (대구한의대학교 한의과대학 방제학교실) ;
  • 김예림 (대구한의대학교 한의과대학 방제학교실) ;
  • 변성희 (대구한의대학교 한의과대학 방제학교실) ;
  • 김상찬 (대구한의대학교 한의과대학 방제학교실)
  • Received : 2023.05.09
  • Accepted : 2023.05.18
  • Published : 2023.05.31
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Abstract

Objectives : Sihosogan-tang (SST) is one of the traditional herbal formula and also one of the Korean medical insurance medicines. It commonly used in the treatment of hepatitis, chronic gastritis, intercostal neuralgia, pleurisy, and depression in East Asia. In the present study, we have demonstrated the anti-inflammatory effects of SST in macrophage cell line. Methods : To investigate mechanism of the anti-inflammatory effect of SST, we examined the productions of nitric oxide (NO) and pro-inflammatory cytokines, and the expressions of inducible NO synthase (iNOS), nuclear factor-κ B (NF-κB) and mitogen-activated protein kinase (MAPK) on RAW 264.7 cells activated by LPS. Results : SST significantly inhibited the expression of iNOS increased by LPS, and also significantly inhibited the production of NO. In addition, SST significantly inhibited pro-inflammatory cytokines such as TNF- α and interleukines. SST inhibited the expression of NF-κB and MAPK activation. Conclusions : These results suggest that SST ameliorates inflammatory response in LPS-activated RAW 264.7 cells through the inhibition of the NF-κB and MAPK pathway. Therefore, this study supplies objective evidence for the anti-inflammatory effect of SST.

Keywords

Acknowledgement

This study was supported by the National Research Foundation of Korea funded by Korea government (MSIP) (Grant No.2018R1A5A2025272)

References

  1. Hwang DY. Bangyakhappyeon. Seoul:Namsandang. 1985:324.
  2. Herbal Formula Textbook Compilation Committee. Herbal Formula Science in Korean Medicine (Special). Paju:Koonja Publisher. 2023:745-747, 1313.
  3. Kim BH. The Effects of Shihosogan-san on Alcohol-induced Muscle Atrophy in Rats. Herbal Formula Science. 2016;24(4):311-321. https://doi.org/10.14374/HFS.2016.24.4.311
  4. Koo LB, Choi DY. Experimental Study on the Recovery Sihosogan-San on the Injury of Liver Rat Induced by Stress. J of Oriental Medical Pathology. 1988;3:63-70.
  5. Ban SH, Choi SH, Ahn KS. The Effect of Sihosogansan, Sihosogansan and Desmodi Herba and Sihosogansan and Curcumac Tuber on the Cholecytolithiasis induced with Gallstone-trigger Diet in Mice. J of Oriental Medical Pathology. 1991;6:145-157.
  6. Jung MH, Lee TH. Anti-stress effects of Sihosogansan in the passive avoidance test and the forced swimming test. Kor. J. Herbology. 2007;22(2):127-135.
  7. Li Z, Zheng HL. Observation of Therapeutic Effect on Depression Treated by Chaihu Shugan Decoction. Journal of Practical Traditional Chinese Medicine. 2009;25(10):652-653.
  8. An SJ, Lee TH. Effect of Chaihushugansan and Chaihuyigantang on the change of Monoamine Contents in the Hypothalamus of Mice induced by the Immobilization Stress. The Korean Journal of Oriental Medical Prescription. 2003;11(1):99-114.
  9. Tan ZH, Yang B, Hu HS, Xie LH, Duan JL. Clinical Observation of Chaihu Shugan Decoction in Treating Functional Dyspepsia. Proceeding of Clinical Medicine. 2010;19(1):41-42.
  10. Lu L, Diao LM, Li H, Liao XQ. Research status of Chaihu Shugan Decoction in treating epilepsy. Chinese Journal of Integrative Medicine on Cardio-cerebrovascular Disease. 2021;19(19):3351-3354.
  11. Nathan C. Points of control on inflammation. Nature. 2002;420:846-852. https://doi.org/10.1038/nature01320
  12. Higuchi M, Higashi N, Taki H, Osawa T. Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine-dependent mechanism acts as synergistically as the major cytolytic mechanism of activated macrophages. J Immunol. 1990;144:1425-1431. https://doi.org/10.4049/jimmunol.144.4.1425
  13. Sharif O, Bolshakov VN, Raines S, Newham P, Perkins ND. Transcriptional profiling of the LPS induced NF-kappaB response in macrophages. BMC Immunol. 2007;8:1-17. https://doi.org/10.1186/1471-2172-8-1
  14. Cinelli MA, Do HT, Miley GP, Silverman RB. Inducible nitric oxide synthase: Regulation, structure, and inhibition. Medicinal research reviews, 2020;40(1):158-189. https://doi.org/10.1002/med.21599
  15. Solt LA, MAY MJ. The IκB kinase complex: master regulator of NF-κB signaling. Immunologic research, 2008;42:3-18. https://doi.org/10.1007/s12026-008-8025-1
  16. Johnson GL, Lapadat R. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science. 2002;298(5600):1911-1912. https://doi.org/10.1126/science.1072682
  17. Abul K. Abbas, Andrew H. Lichman. Cellular and Molecular Immunology (5th Ed). Saunders: Philadelpia, 2003:25, 243-288, 493, 507.
  18. Jeremy S Paige, Samie R Jaffrey. Pharmacologic manipulation of nitric oxide signaling: targeting NOS dimerization and protein-protein interactions. Curr Top Med Chem. 2007;7(1):97-114. https://doi.org/10.2174/156802607779318253
  19. Kubes P. Inducible nitric oxide synthase: a little bit of good in all of us. Gut. 2000;47:6-9. https://doi.org/10.1136/gut.47.1.6
  20. Chiou WF, Chou CJ, Chen CF. Camptothecin suppresses nitric oxide biosynthesis in RAW 264.7 macrophages. Life Sci. 2001;69:625-635. https://doi.org/10.1016/S0024-3205(01)01154-7
  21. Wang H, Wu J, Ma L, Bai Y, Liu J. The role of interleukin-1 family in fibrotic diseases. Cytokine. 2023;165:156161.
  22. Aliyu M, Zohora FT, Anka AU, Ali K, Maleknia S, Saffarioun M, Azizi G. Interleukin-6 cytokine: An overview of the immune regulation, immune dysregulation, and therapeutic approach. Int Immunopharmacol. 2022;111:109130.
  23. Tiegs G, Horst AK. TNF in the liver: targeting a central player in inflammation. Semin Immunopathol. 2022;44(4):445-459. https://doi.org/10.1007/s00281-022-00910-2
  24. Jang WY, Hwang JY, Cho JY. Ginsenosides from Panax ginseng as Key Modulators of NF-κB Signaling Are Powerful Anti-Inflammatory and Anticancer Agents. Int. J. Mol. Sci. 2023;24:6119.
  25. Johnson GL, Lapadat R. Mitogen-activated protein kinase pathways mediated by ERK, JNK, and p38 protein kinases. Science. 2002;298:1911-1912. https://doi.org/10.1126/science.1072682
  26. Huang P, Han J, Hui L. MAPK signaling in inflammation-associated cancer development. Protein Cell. 2010;1(3):218-226. https://doi.org/10.1007/s13238-010-0019-9
  27. Kim SY, Park SM, Hwangbo M, Lee JR, Byun SH, Ku SK, Cho IJ, Kim SC, Jee SY, Park SJ. Cheongsangbangpung-tang ameliorated the acute inflammatory response via the inhibition of NF-κB activation and MAPK phosphorylation. BMC Complement Altern Med. 2017;17(1):46.
  28. Park SM, Kim SC, Byun SH, Kim YW, Chi IJ. Inhibitory effect of Mori Folium ethanol extract on pro-inflammatory mediator in lipopolysaccharide - activated RAW 264.7 cells. Kor. J. Herbology. 2012;27(3):31-38. https://doi.org/10.6116/kjh.2012.27.3.31
  29. Kim BW. Anti-inflammatory Effect of Jakyakgamchotang. Korean J. Orient. Int. Med. 2010:31(2)365-371.