Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Unraveling Biohydrogen Production and Sugar Utilization Systems in the Electricigen Shewanella marisflavi BBL25

  • Sang Hyun Kim (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Hyun Joong Kim (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Su Hyun Kim (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Hee Ju Jung (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Byungchan Kim (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Do-Hyun Cho (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Jong-Min Jeon (Green and Sustainable Materials R&D Department, Research Institute of Clean Manufacturing System, Korea Institute of Industrial Technology (KITECH)) ;
  • Jeong-Jun Yoon (Green and Sustainable Materials R&D Department, Research Institute of Clean Manufacturing System, Korea Institute of Industrial Technology (KITECH)) ;
  • Sang-Hyoun Kim (School of Civil and Environmental Engineering, Yonsei University) ;
  • Jeong-Hoon Park (Sustainable Technology and Wellness R&D Group, Korea Institute of Industrial Technology (KITECH)) ;
  • Shashi Kant Bhatia (Department of Biological Engineering, College of Engineering, Konkuk University) ;
  • Yung-Hun Yang (Department of Biological Engineering, College of Engineering, Konkuk University)
  • Received : 2022.12.12
  • Accepted : 2023.02.02
  • Published : 2023.05.28
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Abstract

Identification of novel, electricity-producing bacteria has garnered remarkable interest because of the various applications of electricigens in microbial fuel cell and bioelectrochemical systems. Shewanella marisflavi BBL25, an electricity-generating microorganism, uses various carbon sources and shows broader sugar utilization than the better-known S. oneidensis MR-1. To determine the sugar-utilizing genes and electricity production and transfer system in S. marisflavi BBL25, we performed an in-depth analysis using whole-genome sequencing. We identified various genes associated with carbon source utilization and the electron transfer system, similar to those of S. oneidensis MR-1. In addition, we identified genes related to hydrogen production systems in S. marisflavi BBL25, which were different from those in S. oneidensis MR-1. When we cultured S. marisflavi BBL25 under anaerobic conditions, the strain produced 427.58 ± 5.85 µl of biohydrogen from pyruvate and 877.43 ± 28.53 µl from xylose. As S. oneidensis MR-1 could not utilize glucose well, we introduced the glk gene from S. marisflavi BBL25 into S. oneidensis MR-1, resulting in a 117.35% increase in growth and a 17.64% increase in glucose consumption. The results of S. marisflavi BBL25 genome sequencing aided in the understanding of sugar utilization, electron transfer systems, and hydrogen production systems in other Shewanella species.

Keywords

Acknowledgement

This study was supported by the Research Program to solve social issues with the National Research Foundation of Korea (NRF), funded by the Ministry of Science and ICT [Grant No. 2017M3A9E4077234], National Research Foundation of Korea (NRF) [Grant Nos. NRF-2022M3I3A1082545, NRF-2022R1A2C2003138 and NRF-2019M3E6A1103979]. This study was also supported by the R&D Program of MOTIE/KEIT [Grant No. 20016324]. The authors also acknowledge the KU Research Professor Program of Konkuk University, Seoul, South Korea.

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