International Journal of Stem Cells

  • 제16권1호
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  • Pages.93-107
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  • 2023
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  • 2005-3606(pISSN)
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  • 2005-5447(eISSN)
한국줄기세포학회 (Korean Society for Stem Cell Research)
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Tracking of Stem Cells from Human Exfoliated Deciduous Teeth Labeled with Molday ION Rhodamine-B during Periodontal Bone Regeneration in Rats

  • Nan Zhang (The Institute for Tissue Engineering and Regenerative Medicine, Liaocheng People's Hospital) ;
  • Li Xu (The Institute for Tissue Engineering and Regenerative Medicine, Liaocheng People's Hospital) ;
  • Hao Song (The Institute for Tissue Engineering and Regenerative Medicine, Liaocheng People's Hospital) ;
  • Chunqing Bu (Department of MRI, Liaocheng People's Hospital) ;
  • Jie Kang (Department of Stomatology, Liaocheng People's Hospital) ;
  • Chuanchen Zhang (Department of MRI, Liaocheng People's Hospital) ;
  • Xiaofei Yang (Department of Orthopedics, Liaocheng People's Hospital) ;
  • Fabin Han (The Institute for Tissue Engineering and Regenerative Medicine, Liaocheng People's Hospital)
  • 투고 : 2021.10.23
  • 심사 : 2022.06.17
  • 발행 : 2023.02.28
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초록

Background and Objectives: Chronic periodontitis can lead to alveolar bone resorption and eventually tooth loss. Stem cells from exfoliated deciduous teeth (SHED) are appropriate bone regeneration seed cells. To track the survival, migration, and differentiation of the transplanted SHED, we used super paramagnetic iron oxide particles (SPIO) Molday ION Rhodamine-B (MIRB) to label and monitor the transplanted cells while repairing periodontal bone defects. Methods and Results: We determined an appropriate dose of MIRB for labeling SHED by examining the growth and osteogenic differentiation of labeled SHED. Finally, SHED was labeled with 25 ㎍ Fe/ml MIRB before being transplanted into rats. Magnetic resonance imaging was used to track SHED survival and migration in vivo due to a low-intensity signal artifact caused by MIRB. HE and immunohistochemical analyses revealed that both MIRB-labeled and unlabeled SHED could promote periodontal bone regeneration. The colocalization of hNUC and MIRB demonstrated that SHED transplanted into rats could survive in vivo. Furthermore, some MIRB-positive cells expressed the osteoblast and osteocyte markers OCN and DMP1, respectively. Enzyme-linked immunosorbent assay revealed that SHED could secrete protein factors, such as IGF-1, OCN, ALP, IL-4, VEGF, and bFGF, which promote bone regeneration. Immunofluorescence staining revealed that the transplanted SHED was surrounded by a large number of host-derived Runx2- and Col II-positive cells that played important roles in the bone healing process. Conclusions: SHED could promote periodontal bone regeneration in rats, and the survival of SHED could be tracked in vivo by labeling them with MIRB. SHED are likely to promote bone healing through both direct differentiation and paracrine mechanisms.

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과제정보

The present study was supported by the Natural Science Youth Foundation of China (grant no. 81800980), the Natural Science Youth Training Foundation of Shandong Province (grant no. ZR2019PC017) and Medical and Health Technology Development Project of Shandong Province (grant no. 2018WS422).

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