Journal of the Korean Magnetic Resonance Society (한국자기공명학회논문지)

Korean Magnetic Resonance Society (한국자기공명학회)
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H-1, C-13, and N-15 resonance assignments of ENOD40B, a plant peptide hormone

  • Received : 2023.05.16
  • Accepted : 2023.06.10
  • Published : 2023.06.20
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Abstract

t ENOD40B, a plant peptide hormone, was doubly labeled with C-13 and N-15 by recombinant production in Escherichia coli. The peptide was prepared by affinity chromatography followed by protease cleavage and reverse-phase chromatography. To elucidate the mode of action against its receptor, sucrose synthase, we proceeded to assign the backbone and side-chain resonances using a set of double and triple resonance experiments. This result will be used to determine the three-dimensional structure of the peptide at its bound state as well as to observe the chemical shift changes upon binding.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF-2021R1F1A1046500), and the faculty research fund of Sejong University in 2023. The author thanks Dr. Marco Tonelli of the NMRFAM for his technical assistance.

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