Journal of Korean Society on Water Environment (한국물환경학회지)

Korean Society on Water Environment (한국물환경학회)
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Denitrification Performance and Bacterial Community Structure of Methanol and Mixed Carbon Sources

메탄올과 혼합 외부탄소원의 탈질성능과 박테리아 군집 비교

  • Suin Park (Department of Civil and Environmental Engineering, Pusan National University) ;
  • Junbeom Jeon (Department of Civil and Environmental Engineering, Pusan National University) ;
  • Minkyu Choi (Water.Environment Research Center, Korea Dyeing and Finishing Technology Institute (DYETEC)) ;
  • Sungjin Kim (Water.Environment Research Center, Korea Dyeing and Finishing Technology Institute (DYETEC)) ;
  • Sanghun Lee (Water.Environment Research Center, Korea Dyeing and Finishing Technology Institute (DYETEC)) ;
  • Taeho Lee (Department of Civil and Environmental Engineering, Pusan National University) ;
  • Sanghyun Jeong (Department of Civil and Environmental Engineering, Pusan National University) ;
  • Hyokwan Bae (Department of Urban and Environmental Engineering, Ulsan National Institute of Science and Technology)
  • 박수인 (부산대학교 사회환경시스템공학과) ;
  • 전준범 (부산대학교 사회환경시스템공학과) ;
  • 최민규 (다이텍연구원 물.환경연구단) ;
  • 김성진 (다이텍연구원 물.환경연구단) ;
  • 이상헌 (다이텍연구원 물.환경연구단) ;
  • 이태호 (부산대학교 사회환경시스템공학과) ;
  • 정상현 (부산대학교 사회환경시스템공학과) ;
  • 배효관 (울산과학기술원 도시환경공학과)
  • Received : 2023.01.03
  • Accepted : 2023.01.30
  • Published : 2023.01.30
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Abstract

Denitrifying bacteria convert nitrate to nitrogen gas using an external carbon source as an electron donor. The external carbon source affects the denitrification performance and bacterial community structure. Although methanol is a cheap and effective external carbon source, the addition of diverse carbon sources may improve the total nitrogen removal rate and biomass characteristics, such as settleability. In this study, denitrifying reactions were performed using solely methanol and mixed carbon sources of methanol, glucose, and acetate in a sequencing batch reactor. The denitrifying reactor using methanol resulted in a total nitrogen removal rate of 0.39 ± 0.025 kg-N/m3-day while the suspended biomass transformed into dark brown granules. Methyloversatilis discipulorum had the highest predominance at 43.84%. The individual denitrifying biomasses, which were separately enriched with methanol, glucose, and acetate, showed the same total nitrogen removal performance of 0.39 ± 0.016 kg-N/m3-day. However, the addition of mixed carbon sources showed an improved total nitrogen removal rate of 0.42 ± 0.043 kg-N/m3-day, with the domination of Candidatus Saccaribacteria at 25.61%. The denitrifying granules turned pale yellow color. Influent COD/NO3--N ratios of 3.5, 5, and 7.5 exhibited COD/NO3--N consumptions of 4.3 ± 0.4, 4.4 ± 0.8, and 5.2 ± 0.7, and the consistent predominance of Candidatus Saccharibacteria.

Keywords

Acknowledgement

본 연구는 2021학년도 부산대학교 BK21 FOUR 대학원혁신지원사업 지원으로 이루어졌습니다.

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