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Pretreatment and Rapid Detection Methods for Wastewater-Based Epidemiology

하수역학 구축을 위한 시료 전처리 기술과 신속검출기술

  • Lee Jai-Yeop (Department of Environment Research, Korea Institute of Civil Engineering and Building Technology) ;
  • Lee Bokjin (Department of Environment Research, Korea Institute of Civil Engineering and Building Technology) ;
  • Jesmin Akter (Department of Environment Research, Korea Institute of Civil Engineering and Building Technology) ;
  • Ahn Chang Hyuk (Department of Environment Research, Korea Institute of Civil Engineering and Building Technology) ;
  • Kim Ilho (Department of Environment Research, Korea Institute of Civil Engineering and Building Technology)
  • 이재엽 (한국건설기술연구원 환경연구본부) ;
  • 이복진 (한국건설기술연구원 환경연구본부) ;
  • 제스민아터 (한국건설기술연구원 환경연구본부) ;
  • 안창혁 (한국건설기술연구원 환경연구본부) ;
  • 김일호 (한국건설기술연구원 환경연구본부)
  • Received : 2022.11.22
  • Accepted : 2023.01.12
  • Published : 2023.01.30

Abstract

Wastewater Based Epidemiology (WBE) provides useful information not only on the use of illegal drugs in the community, but also on the presence of hygiene and health products and infectious pathogens in sewage facilities. As a consequence of the SARS-CoV-19 virus epidemic in 2019, monitoring the status of the infection is of utmost importance. SARS-CoV-19 was also detected in sewage, and the number and trend of infections in the community suggest that the application of the WBE system would be useful and appropriate. This study introduces a pre-treatment concentration method including viruses in sewage samples. A total of seven methods which were subdivided into methods for adsorption-extraction, ultra-filtration, PEG precipitation, and ultra-centrifugation, and the results for analyzing the recovery rates were included. Meanwhile, it is necessary to pay attention to rapid detection technologies which analyze infectious pathogens at the site of sewage facilities. These can include ELISA, FTIR, SERS, and biosensor based on the detection principle, and the characteristics, advantages, and disadvantages of each were summarized herein. If rapid detection technologies and accurate quantitative analyses are further developed, the use of sewage mechanics in response to pandemic viruses is expected to expand further.

Keywords

Acknowledgement

The authors are very grateful for the funds [Project, NRF-2021K1A4A8A01079319] provided by the "National R&D Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science and ICT".

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