Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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Pectobacterium brasiliense as a Causative Agent for Soft Rot of Radish in Korea

  • Kyoung-Taek Park (School of Applied Biosciences, Kyungpook National University) ;
  • Soo-Min Hong (School of Applied Biosciences, Kyungpook National University) ;
  • Chang-Gi Back (Horticultural and Herbal Crop Environment Division, National Institute of Horticultural and Herbal Science, Rural Development Administration) ;
  • In-Kyu Kang (Department of Horticultural Science, Kyungpook National University) ;
  • Seung-Yeol Lee (School of Applied Biosciences, Kyungpook National University) ;
  • Leonid N. Ten (School of Applied Biosciences, Kyungpook National University) ;
  • Hee-Young Jung (School of Applied Biosciences, Kyungpook National University)
  • Received : 2022.11.23
  • Accepted : 2022.12.28
  • Published : 2023.03.31
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Abstract

In October 2021, soft rot disease seriously affected radish crop in Dangjin, Chungcheongnam-do, Korea. The infected radishes were stunted and turned dark green, with yellowish leaf foliage. A slimy, wet, and decayed pith region was observed in the infected roots. The bacterial strain KNUB-03-21 was isolated from infected roots. The biochemical and morphological characteristics of the isolate were similar to those of Pectobacterium brasiliense. Phylogenetic analysis based on the sequences of the 16S rRNA region and the concatenated DNA polymerase III subunit tau (dnaX), leucine-tRNA ligase (leuS), and recombinase subunit A (recA) genes confirmed that the isolate is a novel strain of P. brasiliense. Artificial inoculation of radish with P. brasiliense KNUB-03-21 resulted in soft rot symptoms similar to those observed in infected radish in the field; subsequently, P. brasiliense KNUB-03-21 was reisolated and reidentified. To our knowledge, this is the first report of P. brasiliense as a causal pathogen of radish soft rot in Korea.

Keywords

Acknowledgement

This work was supported by a 2022 fund by Research of Animal and Plant Quarantine Agency, South Korea.

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