Journal of dental hygiene science (치위생과학회지)

The Korean Society of Dental Hygiene Science (한국치위생과학회)
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Silver Diamine Fluoride Compound for Dental Caries and Its Characterisation Using Microscopic Computed Tomography and Nanoindentation

  • So-Youn An (Department of Pediatric Dentristry & Wonkwang Bone Regeneration Research Institute, College of Dentistry, Wonkwang University) ;
  • Myung-Jin Lee (Department of Dental Hygiene, Baekseok University) ;
  • Min-Kyung Kang (Department of Dental Hygiene, Hanseo University) ;
  • Youn-Soo Shim (Department of Dental Hygiene, Sunmoon University)
  • Received : 2023.02.27
  • Accepted : 2023.03.23
  • Published : 2023.03.31
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Abstract

Background: In our study, a silver diamine fluoride (SDF) compound for the treatment of dental caries was synthesized to characterize its remineralization activity upon direct application to deciduous teeth. This study aimed to use microscopic computed tomography (microCT) and nanoindentation to evaluate whether SDF composite application could effectively arrest dental caries in five exfoliated primary molars. Methods: Carious teeth were extracted and visually examined using quantitative photofluorescence devices (Qraycam and QraypenTM). After performing microCT, the SDF composite was applied to the teeth according to the manufacturer's instructions. The researchers exchanged and precipitated the irritant saliva once daily for 1 week. The teeth were sectioned longitudinally through the centers of the mesial and distal surfaces, embedded, polished, and measured using nanoindentation. Thereafter, microCT was repeated. Statistical analyses were performed using GraphPad Prism software. Results: Following SDF composite application, a remineralized layer was observed on microCT images, and the hardness increased when measured using nanoindentation. We found that demineralized enamel presented with an increased number of irregular crystals in the deep carious lesion group compared with those in the shallow carious lesion group, resulting in a rougher surface. Conclusion: The SDF composite may be used for remineralization of early caries and cessation of advanced caries in primary molars.

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References

  1. Ionescu AC, Cazzaniga G, Ottobelli M, Ferracane JL, Paolone G, Brambilla E: In vitro biofilm formation on resin-based composites cured under different surface conditions. J Dent 77: 78-86, 2018. https://doi.org/10.1016/j.jdent.2018.07.012
  2. Cazzaniga G, Ottobelli M, Ionescu AC, et al.: In vitro biofilm formation on resin-based composites after different finishing and polishing procedures. J Dent 67: 43-52, 2017. https://doi.org/10.1016/j.jdent.2017.07.012
  3. Silverstone LM, Wefel JS: The effect of remineralization on artificial caries-like lesions and their crystal content. J Cryst Growth 53: 148-159, 1981. https://doi.org/10.1016/0022-0248(81)90061-0
  4. Abou Neel EA, Aljabo A, Strange A, et al.: Demineralization-remineralization dynamics in teeth and bone. Int J Nanomedicine 11: 4743-4763, 2016. https://doi.org/10.2147/IJN.S107624
  5. Horst JA, Tanzer JM, Milgrom PM: Fluorides and other preventive strategies for tooth decay. Dent Clin North Am 62: 207-234, 2018. https://doi.org/10.1016/j.cden.2017.11.003
  6. Kanduti D, Sterbenk P, Artnik B: Fluoride: a review of use and effects on health. Mater Sociomed 28: 133-137, 2016. https://doi.org/10.5455/msm.2016.28.133-137
  7. Arifa MK, Ephraim R, Rajamani T: Recent advances in dental hard tissue remineralization: a review of literature. Int J Clin Pediatr Dent 12: 139-144, 2019. https://doi.org/10.5005/jp-journals-10005-1603
  8. Walsh T, Worthington HV, Glenny AM, Marinho VC, Jeroncic A: Fluoride toothpastes of different concentrations for preventing dental caries. Cochrane Database Syst Rev 3: CD007868, 2019. https://doi.org/10.1002/14651858.CD007868.pub3
  9. Soares RC, da Rosa SV, Moyses ST, et al.: Methods for prevention of early childhood caries: overview of systematic reviews. Int J Paediatr Dent 31: 394-421, 2021. https://doi.org/10.1111/ipd.12766
  10. Wright JT, White A: Silver diamine fluoride: changing the caries management paradigm and potential societal impact. N C Med J 78: 394-397, 2017. https://doi.org/10.18043/ncm.78.6.394
  11. Burgess JO, Vaghela PM: Silver diamine fluoride: a successful anticarious solution with limits. Adv Dent Res 29: 131-134, 2018. https://doi.org/10.1177/0022034517740123
  12. Kim MR, Lee NY, Jih MK, Kim JW, Choi WS, Lee SH: Comparison of the remineralization effect of newly-developed fluoride agents according to the depth of early carious lesions. Oral Biol Res 44: 93-101, 2020. https://doi.org/10.21851/obr.44.03.202009.93
  13. Gao SS, Zhao IS, Hiraishi N, et al.: Clinical trials of silver diamine fluoride in arresting caries among children: a systematic review. JDR Clin Trans Res 1: 201-210, 2016. https://doi.org/10.1177/2380084416661474
  14. An SY, Park SY, Shim YS: Comparison between QraypenTM imaging and the conventional methods of visual inspection and periapical radiography for proximal caries detection in primary molars: an in vivo study. J Dent Hyg Sci 16: 349-354, 2016. https://doi.org/10.17135/jdhs.2016.16.5.349
  15. Chu CH, Mei ML, Lo EC: Use of fluorides in dental caries management. Gen Dent 58: 37-43; quiz 44-5, 79-80, 2010.
  16. Li X, Bhushan B: A review of nanoindentation continuous stiffness measurement technique and its applications. Mater Charact 48: 11-36, 2002. https://doi.org/10.1016/S1044-5803(02)00192-4
  17. Oliver WC, Pharr GM: An improved technique for determining hardness and elastic modulus using load and displacement sensing indentation experiments. J Mater Res 7: 1564-1583, 1992. https://doi.org/10.1557/JMR.1992.1564
  18. Swain MV, Xue J: State of the art of Micro-CT applications in dental research. Int J Oral Sci 1: 177-188, 2009. https://doi.org/10.4248/IJOS09031
  19. Neboda C, Anthonappa RP, King NM: Tooth mineral density of different types of hypomineralised molars: a micro-CT analysis. Eur Arch Paediatr Dent 18: 377-383, 2017. https://doi.org/10.1007/s40368-017-0306-8
  20. Beltran-Aguilar ED, Goldstein JW, Lockwood SA: Fluoride varnishes. A review of their clinical use, cariostatic mechanism, efficacy and safety. J Am Dent Assoc 131: 589-596, 2000. https://doi.org/10.14219/jada.archive.2000.0232
  21. Goldberg M, Kulkarni AB, Young M, Boskey A: Dentin: structure, composition and mineralization. Front Biosci (Elite Ed) 3: 711-735, 2011. https://doi.org/10.2741/e281
  22. Mei ML, Ito L, Cao Y, Li QL, Lo EC, Chu CH: Inhibitory effect of silver diamine fluoride on dentine demineralisation and collagen degradation. J Dent 41: 809-817, 2013. https://doi.org/10.1016/j.jdent.2013.06.009
  23. Chu CH, Lo EC: Microhardness of dentine in primary teeth after topical fluoride applications. J Dent 36: 387-391, 2008. https://doi.org/10.1016/j.jdent.2008.02.013
  24. Anwar AS, Kumar RK, Prasad Rao VA, Reddy NV, Reshma VJ: Evaluation of microhardness of residual dentin in primary molars following caries removal with conventional and chemomechanical techniques: an in vitro study. J Pharm Bioallied Sci 9(Suppl 1): S166-S172, 2017. https://doi.org/10.4103/jpbs.JPBS_148_17
  25. Jabin Z, Vishnupriya V, Agarwal N, Nasim I, Jain M, Sharma A: Effect of 38% silver diamine fluoride on control of dental caries in primary dentition: a systematic review. J Family Med Prim Care 9: 1302-1307, 2020. https://doi.org/10.4103/jfmpc.jfmpc_1017_19
  26. Mei ML, Chu CH, Lo EC, Samaranayake LP: Fluoride and silver concentrations of silver diammine fluoride solutions for dental use. Int J Paediatr Dent 23: 279-285, 2013. https://doi.org/10.1111/ipd.12005
  27. Rosenblatt A, Stamford TC, Niederman R: Silver diamine fluoride: a caries "silver-fluoride bullet". J Dent Res 88: 116-125, 2009. https://doi.org/10.1177/0022034508329406
  28. Wu L, Geng K, Gao Q: Effects of different anti-caries agents on microhardness and superficial microstructure of irradiated permanent dentin: an in vitro study. BMC Oral Health 19: 113, 2019. https://doi.org/10.1186/s12903-019-0815-4
  29. Toumba KJ, Twetman S, Splieth C, Parnell C, van Loveren C, Lygidakis NΑ: Guidelines on the use of fluoride for caries prevention in children: an updated EAPD policy document. Eur Arch Paediatr Dent 20: 507-516, 2019. https://doi.org/10.1007/s40368-019-00464-2
  30. Tedesco TK, Gimenez T, Floriano I, et al.: Scientific evidence for the management of dentin caries lesions in pediatric dentistry: a systematic review and network meta-analysis. PLoS One 13: e0206296, 2018. https://doi.org/10.1371/journal.pone.0206296
  31. Phantumvanit P, Makino Y, Ogawa H, et al.: WHO global consultation on public health intervention against early childhood caries. Community Dent Oral Epidemiol 46: 280-287, 2018. https://doi.org/10.1111/cdoe.12362
  32. Horst JA, Ellenikiotis H, Milgrom PL: UCSF protocol for caries arrest using silver diamine fluoride: rationale, indications and consent. J Calif Dent Assoc 44: 16-28, 2016. https://doi.org/10.1080/19424396.2016.12220962
  33. Crystal YO, Marghalani AA, Ureles SD, et al.: Use of silver diamine fluoride for dental caries management in children and adolescents, including those with special health care needs. Pediatr Dent 39: 135-145, 2017.