Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Effects of Anemarrhena asphodeloides Extract on Atopic-Dermatitis like Skin Lesions in DNCB-induced Balb/c Mice

DNCB로 유도한 아토피 유사 피부염에 지모 추출물이 미치는 영향

  • Yumi Jang (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Yong-Ung Kim (Department of Pharmaceutical Engineering, College of Biomedical Science, Daegu Haany University) ;
  • Mi Ryeo Kim (Department of Herbal Pharmacology, College of Korean Medicine, Daegu Haany University) ;
  • Hye-Sun Lim (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine) ;
  • Gunhyuk Park (Herbal Medicine Resources Research Center, Korea Institute of Oriental Medicine)
  • 장유미 (한국한의학연구원 한약자원연구센터) ;
  • 김용웅 (대구한의대학교 제약공학과) ;
  • 김미려 (대구한의대학교 한의예과) ;
  • 임혜선 (한국한의학연구원 한약자원연구센터) ;
  • 박건혁 (한국한의학연구원 한약자원연구센터)
  • Received : 2022.12.21
  • Accepted : 2023.01.10
  • Published : 2023.01.31
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Abstract

Under constant environmental pollution, the incidence of Atopic Dermatitis (AD) caused by air pollutants and allergens has increased. AD is an allergy inflammatory skin disease characterized by pruritus, eczema, and skin dryness. In herbal medicine, Anemarrhena asphodeloides (Anemarrhenae Rhizoma; AR) has been utilized to treat Alzheimer's disease, osteoporosis, hypertension, and inflammation. The purpose of study evaluated the effect of AR in a mouse model of 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions. After acclimatization for 5 days, the mice (6-week-old, male Balb/c) were divided into five groups (n=6/group): NC (normal control), DNCB (control), Dex (5 mg·kg-1, p.o.), AR100 (100 mg·kg-1, p.o.), and AR300 (300 mg·kg-1, p.o.). On days 1 and 3, 1% DNCB was applied to the skin and ears. After 4 days, 0.5% DNCB was applied once every 2 days for 2 weeks. Then, skin and ears eczema area and severity index (EASI); skin nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and prostaglandin E2 (PGE2) levels; and plasma immunoglobulin E (IgE) levels were examined. The AR groups showed lower EASI, skin and ear thickness, mast cell count, and IgE levels than the control groups. Moreover, AR reduced iNOS, COX-2, and PGE2 levels. Therefore, AR possesses anti-inflammatory properties and can improve skin damage, indicating its therapeutic potential against AD.

Keywords

Acknowledgement

본 연구는 한국한의학연구원 '지속가능한 한약표준자원 활용기술 개발'(KSN2021320) 사업 및 한국연구재단 '미활용 생물자원의 연구기반 구축 및 활성화 연구: 동물성 자원 중심'(NRF-2020R1I1A2073003)의 지원으로 수행되었다.

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