한국작물학회지 (KOREAN JOURNAL OF CROP SCIENCE)

  • 제68권4호
  • /
  • Pages.294-303
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  • 2023
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  • 0252-9777(pISSN)
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  • 2287-8432(eISSN)
한국작물학회 (The Korean Society of Crop Science)
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Development and Utilization of KASP Markers Targeting the Lipoxygenase Gene in Soybean

  • Seo-Young Shin (Department of Applied Plant Science, Chonnam National University) ;
  • Se-Hee Kang (Department of Applied Plant Science, Chonnam National University) ;
  • Byeong Hee Kang (Department of Applied Plant Science, Chonnam National University) ;
  • Sreeparna Chowdhury (Department of Applied Plant Science, Chonnam National University) ;
  • Won-Ho Lee (Department of Applied Plant Science, Chonnam National University) ;
  • Jeong-Dong Lee (Department of Applied Biosciences, Kyungpook National University) ;
  • Sungwoo Lee (Department of Crop Science, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yu-Mi Choi (National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA) ;
  • Bo-Keun Ha (Department of Applied Plant Science, Chonnam National University)
  • 투고 : 2023.10.16
  • 심사 : 2023.11.06
  • 발행 : 2023.12.01
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초록

Lipoxygenase gives soybeans their grassy flavor, which can disrupt food processing efficiency. This study aimed to identify soybean genotypes with lipoxygenase deficiency among 1,001 soybean accessions and to develop kompetitive allele specific PCR (KASP) markers that can detect lipoxygenase mutations. Three lipoxygenase isozymes (Lox1, Lox2, and Lox3) were analyzed using a colorimetric assay based on a substrate-enzyme reaction. Among the 1,001 accessions examined, two (IT160160 and IT276392) exhibited a deficiency solely in Lox1, and one (IT269984) lacked both Lox1 and Lox2. IT160160 had a 74-bp deletion in exon 8 of Lox1 (Glyma13g347600), whereas IT276392 displayed a missense mutation involving the change of C to A at position 2,880 of Lox1. Moreover, we successfully developed four KASP markers that specifically target Lox1, Lox2, and Lox3 mutations. To validate the Lox1 KASP markers, we used two F2:3 populations generated through a cross between Daepung 2 (lipoxygenase wild type, maternal parent), IT160160, and IT276392 (null Lox1, paternal parent). The results revealed that the Daepung 2 × IT160160 group followed the expected 3:1 ratio according to Mendel's law, whereas the Daepung 2 × IT276392 group did not. Furthermore, a comparison between the colorimetric and KASP marker analyses results revealed a high agreement rate of 96%. KASP markers offer a distinct advantage by allowing the distinction of heterozygous types independent of other variables. As a result, we present an opportunity to expedite the lipoxygenase-deficient cultivar development.

키워드

과제정보

This study was supported by the Cooperative Research Program for Agriculture Science and Technology Development (Project No. PJ014168/RS-2019-RD008897), Rural Development Administration, Republic of Korea.

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