The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology (한방안이비인후피부과학회지)

The Society of Korean Medicine Ophthalmology, Otolaryngology & Dermatology (대한한방안이비인후피부과학회)
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Studies on Skin Anti-aging Effect of Chungpyesagan-tang(CPSGT)

청폐사간탕(淸肺瀉肝湯)의 피부 항노화 효과에 대한 연구

  • Bo-Hee, Jeon (Dept Of Cosmetic Sciences, Semyung University ) ;
  • Young-Chae, Song (Dept. Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Hee-Taek, Kim (Dept. Of Korean Medical Ophtalmology & Otolaryngology & Dermatology, College of Korean Medicine, Semyung University) ;
  • Yong-Min, Kim (Dept. Of Cosmetic Sciences & Beauty Biotechnology, Semyung University)
  • 전보희 (세명대학교 화장품과학과) ;
  • 송영채 (세명대학교 한의과대학 한방안이비인후피부과) ;
  • 김희택 (세명대학교 한의과대학 한방안이비인후피부과) ;
  • 김용민 (세명대학교 화장품뷰티생명공학부)
  • Received : 2023.01.09
  • Accepted : 2023.02.08
  • Published : 2023.02.25
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Abstract

Objectives : This study was conducted to confirm the skin anti-aging effect of Chungpyesagan-tang(CPSGT) extract. Methods : We performed MMT assay to confirm the cytotoxicity of CPSGT. After inducing matrix metalloproteinase-1(MMP-1) with tumor necrosis factor-α(TNF-α), we investigated mRNA expression and protein secretion of MMP-1 by real-time RT-PCR and ELISA. In addition, we measured the protein expression of mitogen-activated protein kinases(MAPKs) and transcription factors by western blot. Results : CPSGT was not cytotoxic at 25-800㎍/㎖. The mRNA expression and protein secretion of MMP-1 decreased when treated with CPSGT. The protein expression of p-ERK, p-JNK, p-p38 was decreased by CPSGT. In addition, the protein expression of p-c-jun and p-NF-κB, which are transcription factors, were also decreased. Conclusion : This suggests that CPSGT can inhibit MMP-1 and thus be a potential anti-aging substance.

Keywords

Acknowledgement

본 과제는 2022년도 교육부의 재원으로 한국연구재단의 지원을 받아 수행된 지자체-대학 협력기반 지역혁신 사업의 결과임(2021RIS-001).

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