Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Analysis of the Effectiveness of Garlic on Gastrointestinal motility disorders using a network pharmacological method

네트워크 약리학 방법을 이용한 위장관 운동성 장애 관련 마늘의 효능 분석

  • Na Ri Choi (Department of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University) ;
  • Byung Joo Kim (Department of Longevity and Biofunctional Medicine School of Korean Medicine, Pusan National University)
  • 최나리 (부산대학교 한의학전문대학원 양생기능의학교실) ;
  • 김병주 (부산대학교 한의학전문대학원 양생기능의학교실)
  • Received : 2023.09.01
  • Accepted : 2023.11.09
  • Published : 2023.11.30
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Abstract

Objectives : The purpose of this study was to explore the compounds, targets and related diseases of garlic by the approaches of network pharmacology and bioinformatics in traditional chinese medicine. Methods : We investigated components and their target molecules of garlic using SymMap and TCMSP and they were compared with analysis platform. Results : 56 potential compounds were identified in garlic, 26 of which contained target information, and it was found that these 26 compounds and 154 targets interact with each other through a combination of 243 compounds. In addition, Apigenin was linked to the most targeted gene (78) in 26 compounds, followed by Kaempferol (61 genes), Nicotic Acid (14 genes), Geraniol (11 genes), Eee (10 genes), and Sobrol A (9 genes). Among 56 potential compounds, three compounds (Kaempferol, Dipterocarpol, and N-Methyl cytisine) corresponded to the active compound by screening criterion Absorption, Distribution, Metabolism, Excretion (ADME). In addition, 12 compounds in 56 potential compounds were associated with gastrointestinal (GI) motility disorder. Among them, Kaempferol was a compound that met the ADME parameters and the rest were potential compounds that did not meet. Also, Kaempferol was closely related to GI motility disorder, indicating that this Kaempferol could be a candidate for potential medical efficacy. Conclusions : It shows the relationship between the compound of garlic, an herbal supplement, and the biological process associated with GI motility disorder. These results are thought to help develop strategies for treating GI motility disorders.

Keywords

Acknowledgement

이 논문은 부산대학교 기본연구지원사업 (2년)에 의하여 연구되었다.

References

  1. Pare P, Ferrazzi S, Thompson WG, Irvine EJ, Rance L. An epidemiological survey of constipation in canada: Definitions, rates, demographics, and predictors of health care seeking. Am J Gastroenterol. 2001;96:3130-3137. https://doi.org/10.1111/j.1572-0241.2001.05259.x
  2. El-Serag HB, Talley NJ. Health-related quality of life in functional dyspepsia. Aliment Pharmacol Ther. 2003;18:387-393. https://doi.org/10.1046/j.1365-2036.2003.01706.x
  3. Huizinga JD. Gastrointestinal peristalsis: Joint action of enteric nerves, smooth muscle, and interstitial cells of Cajal. Microsc Res Tech. 1999;47:239-247. https://doi.org/10.1002/(SICI)1097-0029(19991115)47:4<239::AID-JEMT3>3.0.CO;2-0
  4. Huizinga JD, Lammers WJ. Gut peristalsis is governed by a multitude of cooperating mechanisms. Am J Physiol Gastrointest Liver Physiol. 2009;296:G1-G8.  https://doi.org/10.1152/ajpgi.90380.2008
  5. http://www.whosaeng.com/119179 
  6. Kim JS, Lee KJ, Kim JH, Hahm KB, Cho SW. Functional gastrointestinal disorders in patients referred to specialist gastroenterologists in a tertiary hospital. Korean J Gastrointest Motil. 2004; 10:111-117.
  7. Lee OY. Gastrointestinal Motility Modulating Drugs. J Korean Med Assoc. 2009;52:920-927. https://doi.org/10.5124/jkma.2009.52.9.920
  8. Son OG, Seo BI, Lee SH, Park SJ. The Study of DNA markers to identify of Allium sativum L. Kor J Herbology. 201;29:27-33.  https://doi.org/10.6116/kjh.2014.29.1.27
  9. Kimura S, Tung YC, Pan MH, Su NW, Lai YJ, Cheng KC. Black garlic: A critical review of its production, bioactivity, and application. J Food Drug Anal. 2017;25:62-70.  https://doi.org/10.1016/j.jfda.2016.11.003
  10. Moon SB, Choi NR, Kim JN, Kwon MJ, Kim BS, Ha KT, et al. Effects of black garlic on the pacemaker potentials of interstitial cells of Cajal in murine small intestine in vitro and on gastrointestinal motility in vivo. Anim Cells Syst (Seoul). 2022;26:37-44.  https://doi.org/10.1080/19768354.2022.2049640
  11. Ru J, Li P, Wang J, Zhou W, Li B, Huang C, et al. TCMSP: A database of systems pharmacology for drug discovery from herbal medicines. J Cheminform. 2014;6:13. 
  12. Kong Y, Hao M, Chen A, Yi T, Yang K, Li P, Wang Y, Li P, Jia X, Qin H, Qi Y, Ji J, Jin J, Hua Q, Tai J. SymMap database and TMNP algorithm reveal Huanggui Tongqiao granules for Allergic rhinitis through IFN-mediated neuroimmuno-modulation. Pharmacol Res. 2022;185:106483. 
  13. Alamri MA, Tahir Ul Qamar M. Network pharmacology based virtual screening of Flavonoids from Dodonea angustifolia and the molecular mechanism against inflammation. Saudi Pharm J. 2023;31:101802. 
  14. Shannon P, Markiel A, Ozier O, Baliga NS, Wang JT, Ramage D, et al. Cytoscape: A software environment for integrated models of biomolecular interaction networks. Genome Res. 2003;13:2498-2504. https://doi.org/10.1101/gr.1239303
  15. Doncheva NT, Morris JH, Gorodkin J, Jensen LJ. Cytoscape StringApp: Network Analysis and Visualization of Proteomics Data. J Proteome Res. 2019;18:623-632. https://doi.org/10.1021/acs.jproteome.8b00702
  16. Choi NR, Park J, Ko SJ, Kim JN, Choi W, Park JW, et al. Prediction of the Medicinal Mechanisms of Pinellia ternata Breitenbach, a Traditional Medicine for Gastrointestinal Motility Disorders, through Network Pharmacology. Plants (Basel). 2022;11:1348. 
  17. Zhang R, Zhu X, Bai H, Ning K. Network Pharmacology Databases for Traditional Chinese Medicine: Review and Assessment. Front Pharmacol. 2019;10:123. 
  18. Ideker T, Galitski T, Hood, L. A new approach to decoding life: systems biology. Annu Rev Genomics Hum Genet. 2001;2:343-372. https://doi.org/10.1146/annurev.genom.2.1.343
  19. Li S; Fan TP, Jia W, Lu A, Zhang W. Network pharmacology in traditional Chinese medicine. Evid Based Complement Alternat Med. 2014;2014:138460. 
  20. Hopkins AL. (2008). Network pharmacology: the next paradigm in drug discovery. Nat Chem Biol. 2008;4:682-690. https://doi.org/10.1038/nchembio.118
  21. Li S, Zhang B. Traditional Chinese medicine network pharmacology: theory, methodology and application. Chin J Nat Med. 2013;11:110-120. https://doi.org/10.3724/SP.J.1009.2013.00110
  22. Liang X, Li H, Li S. A novel network pharmacology approach to analyse traditional herbal formulae: the Liu-Wei-Di-Huang pill as a case study. Mol Biosyst. 2014;10:1014-1022. https://doi.org/10.1039/C3MB70507B
  23. Agarwal KC. Therapeutic actions of garlic constituents. Med Res Rev. 1996;16:111-124.  https://doi.org/10.1002/(SICI)1098-1128(199601)16:1<111::AID-MED4>3.0.CO;2-5
  24. Kik C, Kahane R, Gebhardt R. Garlic and health. Nutr Metab Cardiovasc Dis. 2001;11:57-65. 
  25. Kyo E, Uda N, Kasuga S, Itakura Y. Immunomodulatory effects of aged garlic extract. J Nutr. 2001;131:1075S-1079S.  https://doi.org/10.1093/jn/131.3.1075S
  26. Hoshino T, Kashimoto N, Kasuga S. Effects of garlic preparations on the gastrointestinal mucosa. J Nutr. 2001;131:1109S-1113S.  https://doi.org/10.1093/jn/131.3.1109S
  27. Bian Y, Lei J, Zhong J, Wang B, Wan Y, Li J, Liao C, He Y, Liu Z, Ito K, Zhang B. Kaempferol reduces obesity, prevents intestinal inflammation, and modulates gut microbiota in high-fat diet mice. J Nutr Biochem. 2022;99:108840. 
  28. Qu Y, Li X, Xu F, Zhao S, Wu X, Wang Y, Xie J. Kaempferol Alleviates Murine Experimental Colitis by Restoring Gut Microbiota and Inhibiting the LPS-TLR4-NF-κB Axis Front Immunol. 2021;12:679897. 
  29. Ruder B, Atreya R, Becker C. Tumour Necrosis Factor Alpha in Intestinal Homeostasis and Gut Related Diseases. Int J Mol Sci. 2019;20:1887. 
  30. Huizinga JD, Chang G, Diamant NE, El-Sharkawy TY. Electrophysiological basis of excitation of canine colonic circular muscle by cholinergic agents and substance P. J Pharmacol Exp Ther. 1984;692-699. 
  31. Inoue R, Chen S. Physiology of muscarinic receptoroperated nonselective cation channels in guinea-pig ileal smooth muscle. EXS. 1993;261-268. 
  32. Wallace JL, Devchand PR. Emerging roles for cyclooxygenase-2 in gastrointestinal mucosal defense. Br J Pharmacol. 2005;145:275-282. https://doi.org/10.1038/sj.bjp.0706201