Journal of Genetic Medicine

Korean Society of Medical Genetics and Genomics (대한의학유전학회)
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Differential microbiota network according to colorectal cancer lymph node metastasis stages

  • Yeuni Yu (Biomedical Research Institute, Pusan National University School of Medicine) ;
  • Donghyun Han (Department of Medicine, Pusan National University School of Medicine) ;
  • Hyomin Kim (Interdisciplinary Program of Genomic Science, Pusan National University) ;
  • Yun Hak Kim (Periodontal Disease Signaling Network Research Center, School of Dentistry, Pusan National University) ;
  • Dongjun Lee (Department of Convergence Medicine, Pusan National University School of Medicine)
  • Received : 2023.11.23
  • Accepted : 2023.12.12
  • Published : 2023.12.31
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Abstract

Purpose: Colorectal cancer (CRC) is a common malignancy worldwide and the second leading cause of cancer-related deaths. In addition, lymph node metastasis in CRC is considered an important prognostic factor for predicting disease recurrence and patient survival. Recent studies demonstrated that the microbiome makes substantial contributions to tumor progression, however, there is still unknown about the microbiome associated with lymph node metastasis of CRC. Here, we first reported the microbial and tumor-infiltrating immune cell differences in CRC according to the lymph node metastasis status. Materials and Methods: Using Next Generation Sequencing data acquired from 368 individuals diagnosed with CRC (N0, 266; N1, 102), we applied the LEfSe to elucidate microbial differences. Subsequent utilization of the Kaplan-Meier survival analysis enabled the identification of particular genera exerting significant influence on patient survival outcomes. Results: We found 18 genera in the N1 group and 3 genera in the N0 group according to CRC lymph node metastasis stages. In addition, we found that the genera Crenobacter (P=0.046), Maricaulis (P=0.093), and Arsenicicoccus (P=0.035) in the N0 group and Cecembia (P=0.08) and Asanoa (P=0.088) in the N1 group were significantly associated with patient survival according to CRC lymph node metastasis stages. Further, Cecembia is highly correlated to tumor-infiltrating immune cells in lymph node metastasized CRC. Concolusion: Our study highlights that tumor-infiltrating immune cells and intratumoral microbe diversity are associated with CRC. Also, this potential microbiome-based oncology diagnostic tool warrants further exploration.

Keywords

Acknowledgement

This work was supported by grants from the Basic Science Research Program through the National Research Foundation of Korea (NRF), funded by the Ministry of Education (NRF-2021R1A2C4001466, 2022R1A5A2027161, and 2021R1A6A3A01086785).

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