Korean Journal of Ecology and Environment (생태와환경)

The Korean Society of Limnology (한국수생태학회)
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Comparison of Biological Responses and Heat Shock Protein 70 Expression in Chironomidae by Exposure UV and O3

UV와 O3 노출에 따른 깔따구류의 생물학적 반응 및 열충격 단백질 70 발현

  • Ji-Hoon Kim (Department of Ocean Integrated Science, Chonnam National University) ;
  • Won-Seok Kim (Department of Ocean Integrated Science, Chonnam National University) ;
  • Jae-Won Park (Department of Ocean Integrated Science, Chonnam National University) ;
  • Bong-Soon Ko (Department of Ocean Integrated Science, Chonnam National University) ;
  • Kiyun Park (Fisheries Science Institute, Chonnam National University) ;
  • Ihn-Sil Kwak (Department of Ocean Integrated Science, Chonnam National University)
  • 김지훈 (전남대학교 해양융합과학과) ;
  • 김원석 (전남대학교 해양융합과학과) ;
  • 박재원 (전남대학교 해양융합과학과) ;
  • 고봉순 (전남대학교 해양융합과학과) ;
  • 박기연 (전남대학교 수산과학연구소) ;
  • 곽인실 (전남대학교 해양융합과학과)
  • Received : 2023.12.02
  • Accepted : 2023.12.29
  • Published : 2023.12.31
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Abstract

UV and O3 are materials used in the water treatment process, and many studies have been reported to remove organic matters, contaminants, and microorganisms. In this study, we were investigated effects of Chirnomidae (Chironomus flaviplumus, Chironomus riparius), which are contamination indicator species to exposure UV and O3 for the survival rate, body color change and gene expression response. The survival rate of C. flaviplumus exposed to UV decreased to about 70% after 24 hours, and C. riparius about 50%. There was no change in the survival rate of C. flaviplumus exposed to O3, and C. riparius decreased to 95% after 10 minutes of exposure, but there was no change during the subsequent exposure time. In addition, UV and O3 exposure to the two species in body color faded in a time-dependent. In the HSP70 gene expression, C. riparius showed an increase in expression after UV exposure compared to the control group, and a significant difference was shown 12 hours after exposure (P<0.05). C. flaviplumus exposed to O3 showed a relatively low expression compared to the control group, and showed a significant difference at 10 minutes and 1 hour after exposure (P<0.05). These results reported the ecotoxicological effects on Chironomidae according to UV and O3 exposure. Therefore, the results of this study can be used as basic data to understand the effects of UV and O3, which are disinfectants used in water treatment plants, on Chirnomidae entering plants.

UV와 O3는 정수처리 공정에 이용되는 물질로 유기물, 오염물질 및 미생물을 제거하는 것으로 많은 연구가 보고되어 있다. 본 연구에서는 저서성 대형무척추동물 중 오염지표 생물이며 실내 사육 중인 깔따구(C. flaviplumus, C. riparius)를 이용하여 UV와 O3 노출에 따른 생존율, 체 Fig. 5. Heatmap of relative mRNA expression of HSP70 in C. riparius and C. flaviplumus exposed to UV and O3. 색 변화 및 열충격 단백질(Heat Shock Protein 70) 발현 변화를 관찰하였다. UV에 노출된 C. flaviplumus의 생존율은 24시간 후 70% 감소하였으며, C. riparius는 50%로 감소하였다. O3에 노출된 C. flaviplumus는 생존율의 변화가 없었으며 C. riparius는 노출 10분 후 95%로 감소하였으나, 이후 노출 시간 동안 변화가 없었다. 또한, 체색 변화에서 두 종에 대한 UV와 O3 노출은 시간 의존적으로 붉은색의 체색이 옅어지는 현상을 관찰하였다. HSP70 유전자 발현에서 C. riparius는 UV 노출 후 대조군에 비해 발현이 증가하였으며, 노출 12시간 후에서 유의한 차이를 보였다(P<0.05). O3에 노출된 C. flaviplumus는 대조군에 비해 상대적으로 낮은 발현이 관찰되었으며, 노출 10분과 1시간 후에서 유의한 차이를 보였다 (P<0.05). 이와 같은 결과는 UV와 O3 노출에 따른 깔따구에 대한 생태독성학적 영향을 보고하였다. 따라서 본 연구 결과는 정수장에서 이용되는 소독물질인 UV와 O3가 정수장 내로 유입되는 깔따구에 주는 영향을 파악할 수 있는 기초자료로서 활용될 수 있을 것이다.

Keywords

Acknowledgement

이 논문은 한국연구재단의 지원 (NRF-2018-R1A6A1A-03024314), 환경부의 재원으로 한국환경산업기술원 수생태계 건강성 확보 기술개발사업의 지원 (2021003050001)과 국립생물자원관 의 지원(20210505793-00)을 받아 수행된 연구임.

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