한국식품영양학회지 (The Korean Journal of Food And Nutrition)

  • 제36권6호
  • /
  • Pages.551-560
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  • 2023
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  • 1225-4339(pISSN)
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  • 2287-4992(eISSN)
한국식품영양학회 (The Korean Society of Food and Nutrition)
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들깨 가식부위별 최적 추출 조건 확립을 위한 항산화 및 항염증 활성 평가

Antioxidant and Anti-Inflammatory Effects on Optimal Extraction Conditions of Different Edible Parts of Perilla (Perilla frutescens L.)

  • 김민영 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 김정인 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 김상우 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 김성업 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 오은영 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 이정은 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 이은수 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 안연주 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 이명희 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과) ;
  • 김춘송 (농촌진흥청 국립식량과학원 남부작물부 밭작물개발과)
  • Min Young Kim (Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Jung In Kim (Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Sang Woo Kim (Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Sungup Kim (Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Eunyoung Oh (Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Jeongeun Lee (Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Eunsoo Lee (Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Yeon Ju An (Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Myoung Hee Lee (Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA) ;
  • Choon-Song Kim (Upland Crop Breeding Research Division, Dept. of Sourthern Area Crop Science, National Institute of Crop Science, RDA)
  • 투고 : 2023.11.13
  • 심사 : 2023.12.07
  • 발행 : 2023.12.31
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초록

This study was performed to investigate antioxidant and anti-inflammatory activities of perilla(Perilla frutescens L.) seed, flower and leaf according to extraction condition. Perilla seed extracts(PSE), perilla flower extracts(PFE), perilla leaf extracts(PLE) was extracted by stirring extraction (STE, 25℃), shaking extraction (SHE, 80℃), and sonication assisted extraction(SAE, 25℃) with 94% ethanol, 60% ethanol and distilled water, followed by analysis of total polyphenol and flavonoid and testing radical scavenging activities. The highest total polyphenol content (5.47, 9.36, 38.58 mg gallic acid equivalent/g), total flavonoid content(5.77, 8.62, 46.44 mg catechin equivalent/g), ABTS(10.68, 19.46, 63.56 mg trolox equivalent/g) and DPPH(6.51, 7.69, 79.73 mg trolox equivalent/g) radical scavenging activity of PSE, PFE and PLE was observed in the HWE with 60% ethanol,. Among the three extraction method, SHE provided the best results for yield, polyphenol, flavonoid content of perilla seed, flower, leaf in comparison to STE or SAE. SHE with 60% ethanol of perilla seed, flower, leaf more effectively inhibited secretion of nitric oxide(NO) and pro-inflammatory cytokine in RAW 264.7 macrophage exposed to LPS compared to other extraction solvent and method. Therefore, these extracts obtained from perilla seed, flower, leaf could be used antioxidant and anti-inflammatory ingredients in the food industry.

키워드

과제정보

본 논문은 농촌진흥청 연구사업(과제번호: PJ01678501) 지원에 의해 이루어진 것입니다.

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