Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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5-bromoprotocatechualdehyde suppresses growth of human lung cancer cells through modulation of ROS and the AKT/MAPK signaling pathway

  • Jusnseong Kim (Jeju Marine Research Center, Korea Institute of Ocean Science and Technology (KIOST)) ;
  • Eun-A Kim (Jeju Marine Research Center, Korea Institute of Ocean Science and Technology (KIOST)) ;
  • Nalae Kang (Jeju Marine Research Center, Korea Institute of Ocean Science and Technology (KIOST)) ;
  • Seong-Yeong Heo (Jeju Marine Research Center, Korea Institute of Ocean Science and Technology (KIOST)) ;
  • Soo-Jin Heo (Jeju Marine Research Center, Korea Institute of Ocean Science and Technology (KIOST))
  • Received : 2023.08.07
  • Accepted : 2023.08.25
  • Published : 2023.12.31
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Abstract

Early-stage lung cancer is the deadliest form of the disease. In this study, we investigated the anticancer activity of 5-bromoprotocatechualdehyde (BPCA) extracted from the seaweed Polysiphonia morrowii Harvey (P. morrowii) in lung cancer H460 cells. We extracted P. morrowii powder thrice with 80% aqueous methanol and separated the extract using high-performance liquid chromatography. We then tested BPCA's effects on cell viability, apoptosis, reactive oxygen species (ROS) generation, and protein expression Our results showed that BPCA inhibited tumor cell growth and ROS production and induced apoptosis through mitogen-activated protein kinase (MAPK) and AKT signaling pathways in lung cancer cells. When BPCA was combined with hydrogen peroxide, ROS production and apoptosis increased even further due to the regulation of AKT signaling and JNK-MAPKs pathways. These findings suggest that BPCA induces lung-cancer-cell death through ROS-mediated phosphorylation in AKT/MAPK signaling. This could lead to the development of new and effective treatments for early-stage lung cancer.

Keywords

Acknowledgement

This research was supported by a research grants from the Korea Institute of Ocean Science and Technology (PEA0125, PEA0121) and was partially supported by the National Research Foundation of Korea(NRF) grant funded by the Korea government (MSIP) (NO. NRF_2017R1C1B2011398).

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