DOI QR코드

DOI QR Code

느타리버섯 푸른곰팡이병에 관여하는 Trichoderma 속균의 동정 및 검출을 위한 PCR 기반 DNA 마커 개발

Development of PCR-based DNA markers for identification and detection of Trichoderma species associated with the green mold disease of oyster mushroom

  • 박명수 (한국농수산대학교 식량작물학부) ;
  • 서건식 (한국농수산대학교 식량작물학부) ;
  • 류재산 (한국농수산대학교 식량작물학부) ;
  • 김민경 (한국농수산대학교 식량작물학부) ;
  • 이용국 (한국농수산대학교 식량작물학부)
  • Park, Myung Soo (Department of Crops and Forestry, Korea National University of Agriculture & Fisheries) ;
  • Seo, Geon Sik (Department of Crops and Forestry, Korea National University of Agriculture & Fisheries) ;
  • Ryu, Jae San (Department of Crops and Forestry, Korea National University of Agriculture & Fisheries) ;
  • Kim, Min Kyung (Department of Crops and Forestry, Korea National University of Agriculture & Fisheries) ;
  • Lee, Yong Kuk (Department of Crops and Forestry, Korea National University of Agriculture & Fisheries)
  • 투고 : 2022.08.29
  • 심사 : 2022.09.23
  • 발행 : 2022.10.20

초록

Trichoderma is known as pathogen caused serious green mold disease on commercial production. T. pleuroti and T. pleuroticola were common species in various mushroom media. Many strains of T. pleuroti, known as aggressive species causing major economic losses in Korea, showed benomyl resistance. Accurate identification and detection of Trichoderma species associated with oyster mushrooms is very important for disease control. We developed species-specific primers for T. pleuroticola, T. pleuroti, T. harzianum, and T. atroviride based on species-specific fragments isolated from amplified fragment length polymorphism analysis. PCR products corresponding to the predicted fragment of 500bp, 230bp, 180bp, and 410bp were amplified from T. pleuroticola, T. pleuroti, T. harzianum, and T. atroviride, respectively. Multiplex PCR assay using species-specific primers quickly and accurately identified and detected T. pleuroti from mushroom media in which various species co-exist. Our results can be useful for the effective control of mushroom disease.

키워드

참고문헌

  1. Avrova AO, Hyman LJ. Toth RL and Toth IK. (2002). Application of amplified fragment length polymorphism fingerprinting for taxonomy and identification of the soft rot bacteria Erwinia carotovora and Erwinia chrysanthemi. Appl Environ Microbio 68: 1499-1508. https://doi.org/10.1128/AEM.68.4.1499-1508.2002
  2. Bunbury-Blanchette AL and Walker AK. (2019). Trichoderma species show biocontrol potential in dual culture and greenhouse bioassays against Fusarium basal rot of onion. Bio Control 130: 127-135. https://doi.org/10.1016/j.biocontrol.2018.11.007
  3. Casasnovas F, Fantini EN, Palazzini JM, Giaj-Merlera G, Chulze SN, Reynoso MM and Torres AM. (2013). Development of amplified fragment length polymorphism (AFLP)-derived specific primer for the detection of Fusarium solani aetiological agent of peanut brown root rot. J Appl Microbiol. 114: 1782-1792. https://doi.org/10.1111/jam.12183
  4. Fletcher JT. (1990). Trichoderma and Penicillium diseases of Agaricus bisporus. A literature review for the Horticultural Development Council. London: ADAS.
  5. Janssen P, Coopman R, Huys G, Swings J, Bleeker M, Vos P, Zabeau M and Kersters K. (1996). Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy. Microbiology, 142: 1881-1893. https://doi.org/10.1099/13500872-142-7-1881
  6. Lee SW and Cho YS. (2021). Historical and current perspective of oyster mushroom (Pleurotus ostreatus) cultivation in South Korea. Asian j adv agric. 17: 33-41.
  7. Ospina-Giraldo MD, Royse DJ, Thon M R, Chen X & Romaine CP. (1998). Phylogenetic relationships of Trichoderma harzianum causing mushroom green mold in Europe and North America to other species of Trichoderma from world-wide sources. Mycologia, 90(1), 76-81. https://doi.org/10.1080/00275514.1998.12026881
  8. Park MS, Bae KS, and Yu SH. (2006). Two new species of Trichoderma associated with green mold of oyster mushroom cultivation in Korea. Mycobiology, 34: 111-113. https://doi.org/10.4489/MYCO.2006.34.3.111
  9. Park MS, Seo GS, Lee KH, Bae KS and Yu SH. (2005a). Morphological and cultural characteristics of Trichoderma spp. associated with green mold of oyster mushroom in Korea. Plant Pathol J. 21: 221-228. https://doi.org/10.5423/PPJ.2005.21.3.221
  10. Park MS, Seo GS, Bae KS and Yu SH. (2005b). Characterization of Trichoderma spp. associated with green mold of oyster mushroom by PCR-RFLP and sequence analysis of ITS regions of rDNA. Plant Pathol J. 21: 229-236. https://doi.org/10.5423/PPJ.2005.21.3.229
  11. Rogers SO and Bendich AJ. (1994). Extraction of total cellular DNA from plants, algae and fungi. In: Gelvin S. B. and R. A. Schilperoort, editors. Plant molecular biology manual. Dordrecht: Springer Netherlands. pp. 183-190.
  12. Rozen S. and Skaletsky H. (2000). Primer3 on the WWW for general users and for biologist programmers. In Bioinformatics methods and protocols. Humana Press, Totowa, NJ. pp. 365-386.
  13. Samuels GJ. (1996). Trichoderma: a review of biology and systematics of the genus. Myco Res. 100: 923-935. https://doi.org/10.1016/S0953-7562(96)80043-8
  14. Samuels GJ. Dodd SL, Gams W, Castlebury LA and Petrini O. (2002). Trichoderma species associated with the green mold epidemic of commercially grown Agaricus bisporus . Mycologia, 94: 146-170. https://doi.org/10.1080/15572536.2003.11833257
  15. Sivasithamparam K and Ghisalberti EL. (1998). Secondary metabolism in Trichoderma and Gliocladium. pp 139-191 in: Trichoderma and Gliocladium, Vol. 1. C. P. Kubicek
  16. Song ES, Kim SY, Noh TH, Cho H, Chae SC and Lee BM. (2014). PCR-based assay for rapid and specific detection of the new Xanthomonas oryzae pv. oryzae K3a race using an AFLP-derived marker. J Microbiol Biotechnol. 24: 732-739. https://doi.org/10.4014/jmb.1311.11005
  17. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, Frijters A, Pot J, Peleman J and Kuiper M . (1995). AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23: 4407-4414. https://doi.org/10.1093/nar/23.21.4407