Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Dehydroglyasperin D Suppresses Melanin Synthesis through MITF Degradation in Melanocytes

  • Baek, Eun Ji (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Ha, Yu-Bin (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Kim, Ji Hye (School of Food Science and Biotechnology, Kyungpook National University) ;
  • Lee, Ki Won (Department of Agricultural Biotechnology, Seoul National University) ;
  • Lim, Soon Sung (Department of Food Science and Nutrition, Hallym University) ;
  • Kang, Nam Joo (School of Food Science and Biotechnology, Kyungpook National University)
  • Received : 2022.07.19
  • Accepted : 2022.07.29
  • Published : 2022.08.28
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Abstract

Licorice (Glycyrrhiza) has been used as preventive and therapeutic material for hyperpigmentation disorders. Previously, we isolated noble compounds including dehydroglyasperin C (DGC), dehydroglyasperin D (DGD) and isoangustone A (IAA) from licorice hexane/ethanol extracts. However, their anti-melanogenic effects and underlying molecular mechanisms are unknown. The present study compared effects of DGC, DGD and IAA on pigmentation in melan-a melanocytes and human epidermal melanocytes (HEMn). DGD exerted the most excellent anti-melanogenic effect, followed by DGC and IAA at non-cytotoxic concentrations. In addition, DGD significantly inhibited tyrosinase activity in vitro cell-free system and cell system. Western blot result showed that DGD decreased expression of microphthalmia-associated transcription factor (MITF), tyrosinase and tyrosinase-related protein-1 (TRP-1) in melan-a cells and HEMn cells. DGD induced phosphorylation of MITF, ERK and Akt signal pathway promoting MITF degradation system. However, DGD did not influence p38 and cAMP-dependent protein kinase (PKA)/CREB signal pathway in melan-a cells. These result indicated that DGD inhibited melanogenesis not only direct regulation of tyrosinase but also modulating intracellular signaling related with MITF level. Collectively, these results suggested a protective role for DGD against melanogenesis.

Keywords

Acknowledgement

This research was supported by Kyungpook National University Development Project Research Fund, 2018.

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