Journal of Applied Biological Chemistry

The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Antioxidant and anti-inflammatory effects of silymarin

실리마린의 항산화 및 항염증 효과

  • Park, Hyun-Bin (Department of Biomedical Science, Cheongju University) ;
  • Kyeong, Inn-Goo (Department of Biomedical Science, Cheongju University) ;
  • Kang, Jung-Hoon (Department of Biomedical Science, Cheongju University)
  • Received : 2022.08.04
  • Accepted : 2022.09.13
  • Published : 2022.09.30
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Abstract

We investigated the antioxidant and anti-inflammatory effects of silymarin. The antioxidant activity was evaluated using 1,1-diphenyl-1-picrylhydrazyl radical (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals scavenging assays. Silymarin scavenged 71% of DPPH radicals and 78% of ABTS radicals at a concentration of 1 mg/mL, respectively. Silymarin effectively inhibited the oxidative damage of DNA, and the oxidative modifications of human serum proteins and Cu,Zn-SOD. Also silymarin effectively inhibited H2O2- and LPS-induced cell death as well as the generation of reactive oxygen species and DNA fragmentation by H2O2 and LPS. The results suggested that silymarin might be an effective natural antioxidant and anti-inflammatory material.

본 연구는 실리마린의 항산화 및 항염증 활성을 알아보고자 하였다. 항산화 활성은 2,2-diphenyl-1-picrylhydrazyl (DPPH)와 2,2'-azino-bis (3-ethylbenzothiazoline5 6-sulfonic acid) (ABTS) 라디칼에 대한 소거능을 측정하여 확인하였다. 실리마린은 1 mg/mL의 농도에서 DPPH 라디칼을 71%, ABTS 라디칼을 78% 소거하여 우수한 항산화 효과를 나타냈다. 실리마린은 DNA의 산화적 손상을 효과적으로 억제하였고 사람 혈청 단백질과 Cu,Zn-SOD의 산화적 변형을 억제하였다. 또한 실리마린은 H2O2와 LPS에 의한 세포사멸, ROS 생성 및 DNA fragmentation을 억제하였다. 본 연구 결과들을 통해 실리마린은 효과적인 천연 항산화 및 항염증 소재로 적용될 수 있음을 제시하였다.

Keywords

Acknowledgement

이 논문은 2011년도 청주대학교 연구장학 지원과 2020년도 교육부의 재원으로 한국연구재단의 지원을 받아 수행된 지자체-대학 협력기반 지역혁신 사업의 지원에 의한 것임.

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