Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Antimicrobial resistance and virulence factors in staphylococci isolated from canine otitis externa

개의 외이도에서 분리한 포도상구균의 항생제 내성 및 병독성 유전자

  • Cho, Jae-Keun (Metropolitan Health & Enviornmental Research Institute) ;
  • Lee, Jung-Woo (Metropolitan Health & Enviornmental Research Institute) ;
  • Kim, Joung-Ok (Metropolitan Health & Enviornmental Research Institute) ;
  • Kim, Jeong-Mi (Metropolitan Health & Enviornmental Research Institute)
  • 조재근 (대구광역시보건환경연구원) ;
  • 이정우 (대구광역시보건환경연구원) ;
  • 김정옥 (대구광역시보건환경연구원) ;
  • 김정미 (대구광역시보건환경연구원)
  • Received : 2022.06.24
  • Accepted : 2022.07.27
  • Published : 2022.09.30
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Abstract

The aim of this study was to investigate the prevalence of antimicrobial resistance and virulence factors in staphylococci isolated from canine otitis externa. A total 295 causative microorganisms were isolated. The most common isolated species were Staphylococcus (S) pseudintermedius (94 isolates) followed by Pseudomonas aeruginosa (60 isolates), S. schleiferi (25 isolates), Escherichia coli (23 isolates) and Proteus mirabilis (20 isolates). Staphylococci isolates were showed high resistance to penicillin (78.6%), erythromycin (55.9%), tetracycline (52.4%), clindamycin (51.7%) and ciprofloxacin (42.8%). Of the 145 staphylococci isolates, 49 (33.8%) methicillin-resistant staphylococci (MRS) were observed, distributed among S. pseudintermedius (n=34), S. schleiferi (n=6), S. epidermis (n=4), S. hominis (n=2), S. aureus, S. caprae and S. saprophyticus (n=1, respectively). Forty-three (87.8%) of 49 MRS and 10 (10.4%) of 96 methicillin-susceptibility staphylococci harbored mecA gene. About 80% of MRS were multidrug-resistant with resistance to at least one antibiotic in three or more antibiotic classes. Resistance genes blaZ (93/114, 81.5%), ermB (35/81, 43.2%), ermC (3/81, 3.7%), aacA-aphD (50/54, 92.5%), tetM (69/76, 90.7%) and tetK (6/76, 7.8%) were detected among resistant isolates. Virulence factors genes lukF and lukS were found in 100%(145/145) and 43.4%(63/145), respectively. Genes encoding ermA, eta, etb and tsst were not detected. To the best of our knowledge, this is the first study which investigated for the presence of genes encoding antimicrobial resistance and staphylococcal toxins in staphylococci isolated from canine otitis externa. A continuous monitoring and surveillance program to prevent antimicrobial resistance in companion animals is demanded.

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