Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Prevalence and antimicrobial susceptibility of Streptococcus species isolated from bovine mastitis

젖소 유방염에서 분리한 Streptococcus 종의 분포 및 항생제 내성 분석

  • Kang, Hye Jeong (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Hong, Serim (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Park, Dasom (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Kim, Ha-Young (Bacterial Disease Division, Animal and Plant Quarantine Agency) ;
  • Moon, Jin-San (Bacterial Disease Division, Animal and Plant Quarantine Agency)
  • 강혜정 (농림축산검역본부 세균질병과) ;
  • 홍세림 (농림축산검역본부 세균질병과) ;
  • 박다솜 (농림축산검역본부 세균질병과) ;
  • 김하영 (농림축산검역본부 세균질병과) ;
  • 문진산 (농림축산검역본부 세균질병과)
  • Received : 2022.08.02
  • Accepted : 2022.08.30
  • Published : 2022.09.30
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Abstract

Streptococcus is one of the major pathogen groups inducing bovine mastitis. The aim of this study was to investigate the antimicrobial resistance patterns of Streptococcus species isolated from bovine mastitis milk samples in Korea from 2016 to 2021. In total, 181 (10.3%) Streptococcal isolates were collected from 1,761 quarter milk samples at 122 farms; S. uberis 39.2% (n=71), S. dysgalactiae 29.3% (n=53), S. equinus 9.9% (n=18), S. suis 6.1% (n=11), S. parauberis 4.4% (n=8), S. lutetiensis 3.9% (n=7), others 7.2% (n=13). However, S. agalactiae was not isolated. The isolates showed the highest resistance rate to tetracycline (55.2%) followed by erythromycin (45.3%) and pirlimycin (36.5%). In contrast, all isolates were susceptible to ceftiofur, cephalothin, penicillin/novobiocin, and only single S. equinus isolate was resistant to both ampicillin and penicillin. Of 181 isolates, 64 (35.4%) were multidrug resistance (MDR). The resistance to pirlimycin of S. uberis (73.2%) was much higher than that of other species (0~36.4%). All S. suis isolates were resistance to tetracycline. S. dysgalactiae showed lower resistance to erythromycin, pirlimycin and tetracycline than S. uberis and S. suis. The rate of MDR was relatively higher among S. uberis (73.2%) than among S. suis (36.4%), S. dysgalactiae (15.1%), others (0%). In conclusion, antimicrobial resistance in Streptococcus spp. should be regularly examined for appropriate therapies because the resistance patterns were various among the individual species.

Keywords

Acknowledgement

본 연구는 농림축산검역본부의 농림축산검역검사기술개발사업(과제명: 젖소 유방염 원인균 및 항생제 감수성 양상 조사, N-1543081-2017-36-01)의 연구비 지원에 의해 수행되었다.

References

  1. 김두. 2006. 주요 유방염 원인균에 대한 대책. J Vet Clin 23: 3-14.
  2. 김재명. 2006. 최근 젖소유방염 발생원인균 및 항균제 내성 양상. J Korean Vet Med Assoc 42: 1110-1118.
  3. Abdi RD, Gillespie BE, Ivey S, Pighetti GM, Almeida RA, Kerro Dego O. 2021. Antimicrobial resistance of major bacterial pathogens from dairy cows with high somatic cell count and clinical mastitis. Animals 11: 131. https://doi.org/10.3390/ani11010131
  4. Abed AH, Menshawy A, Zeinhom M, Hossain D, Khalifa E, Wareth G, Awad MF. 2021. Subclinical mastitis in selected bovine dairy herds in North Upper Egypt: Assessment of prevalence, causative bacterial pathogens, antimicrobial resistance and virulence-associated genes. Microorganisms 9: 1175. https://doi.org/10.3390/microorganisms9061175
  5. Animal and plant quarantine agency (APQA). 2021. National antibiotic use and resistance monitoring in 2020: Animals, livestock and marine products. https://ebook.qia.go.kr/20211022_103546.
  6. Ashraf A, Imran M. 2018. Diagnosis of bovine mastitis: from laboratory to farm. Trop Anim Health Prod 50: 1193-1202. https://doi.org/10.1007/s11250-018-1629-0
  7. Botrel MA, Haenni M, Morignat E, Sulpice P, Madec JY, Calavas D. 2010. Distribution and antimicrobial resistance of clinical and subclinical mastitis pathogens in dairy cows in Rhone-Alpes, France. Foodborne Pathog Dis 7: 479-487. https://doi.org/10.1089/fpd.2009.0425
  8. Cameron M, Saab M, Heider L, McClure JT, Rodriguez-Lecompte JC, Sanchez J. 2016. Antimicrobial susceptibility patterns of environmental streptococci recovered from bovine milk samples in the maritime provinces of Canada. Front Vet Sci 3: 79. https://doi.org/10.3389/fvets.2016.00079
  9. Cheng J, Qu W, Barkema HW, Nobrega DB, Gao J, Liu G, De Buck J, Kastelic JP, Sun H, Han B. 2019. Antimicrobial resistance profiles of 5 common bovine mastitis pathogens in large Chinese dairy herds. J Dairy Sci 102: 2416-2426. https://doi.org/10.3168/jds.2018-15135
  10. Cheng WN, Han SG. 2020. Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments-A review. Asian-Australasian J Anim Sci 33: 1699-1713. https://doi.org/10.5713/ajas.20.0156
  11. Cheong CK, Han HR, Chung GT. 1970. Studies on Epidemiological Investigations and Therapy of Mastitis. Korean J Vet Res 10: 39-45.
  12. CLSI (Clinical and laboratory Standards Institute). 2018. Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals; 4th edition. CLSI supplement VET08. 4th ed. CLSI, Wayne, PA.
  13. CLSI (Clinical and laboratory Standards Institute). 2020. Performance standards for antimicrobial susceptibility testing; 30th edition. CLSI document M100. 30th ed. CLSI, Wayne, PA.
  14. Duse A, Persson-Waller K, Pedersen K. 2021. Microbial aetiology, antibiotic susceptibility and pathogen-specific risk factors for udder pathogens from clinical mastitis in dairy cows. Animals 11: 2113. https://doi.org/10.3390/ani11072113
  15. El Garch F, Youala M, Simjee S, Moyaert H, Klee R, Truszkowska B, Rose M, Hocquet D, Valot B, Morrissey I. 2020. Antimicrobial susceptibility of nine udder pathogens recovered from bovine clinical mastitis milk in Europe 2015-2016: VetPath results. Vet Microbiol 245: 108644. https://doi.org/10.1016/j.vetmic.2020.108644
  16. Guerin-Faublee V, Tardy F, Bouveron C, Carret G. 2002. Antimicrobial susceptibility of Streptococcus spe- cies isolated from clinical mastitis in dairy cows. Int J Antimicrob Agents 19: 219-226. https://doi.org/10.1016/S0924-8579(01)00485-X
  17. Halasa T, Huijps K, Osteras O, Hogeveen H. 2007. Economic effects of bovine mastitis and mastitis management: A review. Vet Q 29: 18-31. https://doi.org/10.1080/01652176.2007.9695224
  18. Kabelitz T, Aubry E, van Vorst K, Amon T, Fulde M. 2021. The role of Streptococcus spp. in bovine mastitis. Microorganisms 9: 1497. https://doi.org/10.3390/microorganisms9071497
  19. Kim SE, Hah DY, Jang EH, Kwon HN, JoSS, Kwon YT, Park DY, Lee KC, Kim JS. 2011. Survey of mastitis management and incidence of mastitis in high somatic cell count of bulk milk at dairy farms in the Gyeongnam. Korean J Vet Serv 34: 349-388.
  20. Kromker V, Leimbach S. 2017. Mastitis treatment-Reduction in antibiotic usage in dairy cows. Reprod Domest Anim 52: 21-29. https://doi.org/10.1111/rda.13032
  21. Lee G, Kang HM, Chung CI, Moon JS. 2007. Antimicrobial susceptibility and genetic characteristics of Streptococcus uberis isolated from bovine mastitis milk. Korean J Vet Res 47: 33-41.
  22. Minst K, Martlbauer E, Miller T, Meyer C. 2012. Streptococcus species isolated from mastitis milk samples in Germany and their resistance to antimicrobial agents. J Dairy Sci 95: 6957-6962. https://doi.org/10.3168/jds.2012-5852
  23. Monistero V, Barberio A, Cremonesi P, Castiglioni B, Morandi S, Lassen DC, Astrup LB, Locatelli C, Piccinini R, Addis MF, Bronzo V, Moroni P. 2021. Genotyping and antimicrobial susceptibility profiling of Streptococcus uberis isolated from a clinical bovine mastitis outbreak in a dairy farm. Antibiotics 10: 644. https://doi.org/10.3390/antibiotics10060644
  24. Nam HM, Kim JM, Lim SK, Jang KC, Jung SC. 2010. Infectious aetiologies of mastitis on Korean dairy farms during 2008. Res Vet Sci 88: 372-374. https://doi.org/10.1016/j.rvsc.2009.12.008
  25. Nam HM, Lim SK, Kang HM, Kim JM, Moon JS, Jang KC, Joo YS, Kang MI, Jung SC. 2009. Antimicrobial resistance of streptococci isolated from mastitic bovine milk samples in Korea. J Vet Diagn Invest 21:698-701 https://doi.org/10.1177/104063870902100517
  26. NMC (National Mastitis Council). 2020. Laboratory Handbook on Bovine Mastitis, 3rd ed. New Prague: NMC, USA.
  27. Pitkala A, Koort J, Bjorkroth J. 2008. Identification and antimicrobial resistance of Streptococcus uberis and Streptococcus parauberis isolated from bovine milk samples. J Dairy Sci 91: 4075-4081. https://doi.org/10.3168/jds.2008-1040
  28. Ruegg PL. 2017. A 100-Year Review: Mastitis detection, management, and prevention. J Dairy Sci 100: 10381-10397. https://doi.org/10.3168/jds.2017-13023
  29. Sharun K, Dhama K, Tiwari R, Gugjoo M, Iqbal Yatoo M, Patel SK, Pathak M, Karthik K, Khurana SK, Singh R. 2021. Advances in therapeutic and managemental approaches of bovine mastitis: a comprehensive review. Vet Q 41: 107-136. https://doi.org/10.1080/01652176.2021.1882713
  30. Song GH, Cho JH, Hong JS. 1975. Survey of Bovine Mastitis in Gyeonggi Province 3. Incidence of Bovine Mastitis in Southern Region of the Han River. Korean J Vet Res 15: 109-114.
  31. Song X, Huang X, Xu H, Zhang C, Chen S, Liu F, Guan S, Zhang S, Zhu K, Wu C. 2020. The prevalence of pathogens causing bovine mastitis and their associated risk factors in 15 large dairy farms in China: an observational study. Vet Microbiol 247: 108757. https://doi.org/10.1016/j.vetmic.2020.108757
  32. Tian XY, Zheng N, Han RW, Ho H, Wang J, Wang YT, Wang SQ, Li HG, Liu HW, Yu ZN. 2019. Antimicrobial resistance and virulence genes of Streptococcus isolated from dairy cows with mastitis in China. Microb Pathog 131: 33-39. https://doi.org/10.1016/j.micpath.2019.03.035
  33. Yun MJ, Yoon S, Lee YJ. 2020. Monitoring and characteristics of major mastitis pathogens from bulk tank milk in Korea. Animals 10: 1562. https://doi.org/10.3390/ani10091562