Journal of the Korean Society of Visualization (한국가시화정보학회지)

The Korean Society of Visualization (한국가시화정보학회)
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Visualization of the physical characteristics of collective myoblast migration upon skeletal muscle injury and regeneration environment

골격근 손상 및 재생 환경에서의 근육 세포 군집 이동의 물리적 특성 가시화

  • Kwon, Tae Yoon (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology) ;
  • Jeong, Hyuntae (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology) ;
  • Cho, Youngbin (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology) ;
  • Shin, Jennifer H. (Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology)
  • Received : 2022.06.24
  • Accepted : 2022.07.26
  • Published : 2022.07.31
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Abstract

Skeletal muscle tissues feature cellular heterogeneity, including differentiated myofibers, myoblasts, and satellite cells. Thanks to the presence of undifferentiated myoblasts and satellite cells, skeletal muscle tissues can self-regenerate after injury. In skeletal muscle regeneration, the collective motions among these cell types must play a significant role, but little is known about the dynamic collective behavior during the regeneration. In this study, we constructed in vitro platform to visualize the migration behavior of skeletal muscle cells in specific conditions that mimic the biochemical environment of injured skeletal muscles. We then visualized the spatiotemporal distribution of stresses arising from the differential collectiveness in the cellular clusters under different conditions. From these analyses, we identified that the heterogeneous population of muscle cells exhibited distinct collective migration patterns in the injury-mimicking condition, suggesting selective activation of a specific cell type by the biochemical cues from the injured skeletal muscles.

Keywords

Acknowledgement

이 논문은 한국연구재단 (NRF-2020M3A9E4039658)의 지원을 받아 수행된 연구임.

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