대한화장품학회지 (Journal of the Society of Cosmetic Scientists of Korea)

  • 제48권2호
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  • Pages.113-121
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  • 2022
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  • 1226-2587(pISSN)
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  • 2288-9507(eISSN)
대한화장품학회 (Society of Cosmetic Scientists of Korea)
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젖산균 발효 구기자(Lycium chinense ) 추출물의 항염증 및 항노화 효능 연구

Anti-inflammation and Anti-aging Effect of Lactic Acid Bacteria Fermented Lycium chinense Extracts

  • 한동근 (대구한의대학교 일반대학원 화장품약리학과) ;
  • 이주성 (대구한의대학교 일반대학원 화장품약리학과) ;
  • 갈격 (대구한의대학교 일반대학원 화장품약리학과) ;
  • 박남완 ((주)네이처보타닉스) ;
  • 김현정 ((주)허니스트) ;
  • 안봉전 (대구한의대학교 화장품약리학과)
  • Han, Dong-Geun (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Lee, Ju-Seong (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Gal, Gyeok (Department of Cosmeceutical Science, Daegu Hanny University) ;
  • Park, Nam-Wan (R&D Center, Nature Botanics.Co., Ltd.) ;
  • Kim, Hyun-Jeong (R&D Center, HONEST.Co., Ltd.) ;
  • An, Bong-Jeon (Department of Cosmeceutical Science, Daegu Hanny University)
  • 투고 : 2022.05.25
  • 심사 : 2022.06.16
  • 발행 : 2022.06.30
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초록

본 연구에서는 발효 과정을 거친 구기자(Lycium chinense) 추출물을 이용하여 항염증 및 항노화 관련 기능성 화장품 소재로서의 활용가치를 검증하고자 nitric oxide (NO) 및 항노화 관련 인자들의 억제 효능을 분석하였다. 물과 70% 에탄올을 용매로 구기자를 추출, 발효, 농축, 동결건조하여 시료를 제조한 다음, MTT assay, NO inhibition activity assay, western blot assay, UPLC 분석을 진행하였다. MTT assay를 통해 세포독성을 측정한 다음, 500 ㎍/mL의 농도를 세포실험에 적용하여 진행하였다. NO 억제 효능의 분석 결과, 발효 구기자 열수 추출물(FLW), 발효 구기자 70% 에탄올 추출물(FLE) 각각 47.96%, 56.71%의 우수한 NO 억제 효능을 나타내었다. Western blot을 통해 주름 관련 단백질인 MMP-1, TRPV-1의 발현양상을 측정한 결과, FLW, FLE 모두 MMP-1, TRPV-1에 대한 발현 억제 효능을 나타내었다. 따라서 발효 구기자 추출물은 항염증 및 항노화 관련 기능성 화장품 소재로서의 높은 활용 가치가 있을 것으로 판단된다.

In this study, fermented Lycium chinense (L. chinense) extract was used to analyze the efficacy of nitric oxide (NO) and anti-aging inhibition to verify its usefulness as a functional cosmetic material. L. chinense was extracted with hydrothermal and 70% ethanol as a solvent, fermented, concentrated, and freeze-dried to prepare a sample, followed by MTT assay, NO inhibition assay, western blot assay, and UPLC analysis.As a result of the analysis of NO inhibitory efficacy, fermented L. chinense water extract (FLW) and fermented L. chinense 70% ethanol extract (FLE) showed excellent NO inhibitory efficacy of 47.96% and 56.71%, respectively. As a result of measuring the expression patterns of the wrinkle-related proteins MMP-1, and TRPV-1 through Western blot, both FLW and FLE confirmed the inhibitory efficacy of MMP-1 and TRPV-1. Based on the results of the experiment, the fermented L. chinense extract is expected to have a high application value as a functional cosmetic material related anti-inflammatory, and anti-aging.

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