Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Establishment of withdrawal time and analysis of dexamethasone residue in milk of intramuscularly dosed cows

우유 중 dexamethasone의 잔류 분석 및 휴약기간 설정 연구

  • Park, Kwangil (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University) ;
  • Jin, Yeung Bae (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University) ;
  • Kim, Woohyun (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University) ;
  • Kim, Suk (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University) ;
  • Lee, Hu-Jang (College of Veterinary Medicine and Institute of Animal Medicine, Gyeongsang National University)
  • 박광일 (경상국립대학교 수의과대학 및 동물의학연구소) ;
  • 진영배 (경상국립대학교 수의과대학 및 동물의학연구소) ;
  • 김우현 (경상국립대학교 수의과대학 및 동물의학연구소) ;
  • 김석 (경상국립대학교 수의과대학 및 동물의학연구소) ;
  • 이후장 (경상국립대학교 수의과대학 및 동물의학연구소)
  • Received : 2022.04.22
  • Accepted : 2022.06.09
  • Published : 2022.06.30
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Abstract

This study investigated dexamethasone (DXM) residues in the milk from intramuscularly dosed dairy cows and established the withdrawal time (WT) of DXM in milk. Eighteen healthy Holstein cows were injected with 20 (DXM-1) or 40 mL (DXM-2) of a drug containing 1 mg/mL of DXM. After administering DXM, milk samples were collected from all cows at 12-hour intervals for five days. The DXM residue concentrations in milk were determined by liquid chromatography-tandem mass spectrometry. The correlation coefficient of the calibration curve was 0.9966, and the limits of detection and quantification (LOQ) were 0.03 and 0.1 ㎍/kg, respectively. The recoveries were 97.0% to 104.0%, and the coefficient of variations was less than 7.22%. After treatment, DXM in DXM-1 was detected above the LOQ in two milk samples at 36 hours and below the LOQ in all milk samples of DXM-2 at 48 hours. Using the WT calculation program WT 1.4, the withdrawal periods of DXM-1 and DXM-2 in milk were established to be two days. In conclusion, the developed analytical method is sensitive and reliable for detecting DXM in milk. The estimated WT of DXM in bovine milk is shorter than the current milk WT recommendation of three days for DXM in lactating dairy cows.

Keywords

Acknowledgement

본 논문은 가축위생방역본부(2020년 동물용의약품 안전관리기준 마련을 위한 잔류성 시험분석)의 지원에 의해 수행되었으며, 이에 감사드립니다.

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