Journal of Mushroom (한국버섯학회지)

The Korean Society of Mushroom Science (한국버섯학회)
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Effect of control measures on the contamination and growth inhibition of Listeria monocytogenes in Flammulina velutipes

팽이버섯 재배 농가에서 Listeria monocytogenes 오염과 성장억제를 위한 관리기술 효과

  • Lee, Ha Kyoung (Department of Food and Nutrition, Kyung Hee University) ;
  • Jeon, Ji Hye (Department of Food and Nutrition, Kyung Hee University) ;
  • Lee, Ji Soo (Department of Food and Nutrition, Kyung Hee University) ;
  • Yoon, Seo Young (Department of Food and Nutrition, Kyung Hee University) ;
  • Kim, Won Young (Honam Mushroom Agricultural Association) ;
  • Yoon, Ki Sun (Department of Food and Nutrition, Kyung Hee University)
  • 이하경 (경희대학교 식품영양학과) ;
  • 전지혜 (경희대학교 식품영양학과) ;
  • 이지수 (경희대학교 식품영양학과) ;
  • 윤서영 (경희대학교 식품영양학과) ;
  • 김원영 (호남버섯 영농조합) ;
  • 윤기선 (경희대학교 식품영양학과)
  • Received : 2022.04.06
  • Accepted : 2022.05.30
  • Published : 2022.06.30
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Abstract

The consumption of Flammulina velutipes mushroom imported from Korea has been associated with the cases of listeriosis in the United States, Canada, and Australia. We investigated the effect of sanitizing the plastic wrapper (used in packaging F. velutipes) with slightly acidic electrolyzed water (SAEW) and ultraviolet C waterproof light-emitting diode (UVC-W-LED) on reducing the Listeria monocytogenes. Further, the effect of UVC-LED on L. monocytogenes growth in F. velutipes at different storage temperatures (2, 4, and 10℃) was determined. The combined (SAEW+UVC-W-LED) treatment for 5-10 min reduced 99.9% of bacterial population from the contaminated plastic wrapper. In addition, the UVC-LED treatment for 3 min reduced the L. monocytogenes concentration in F. velutipes by 0.47 log CFU/g. Moreover, the growth of L. monocytogenes in the treated mushrooms was slower than that of the untreated (control) ones. L. monocytogenes concentration in F. velutipes increased over 3 log CFU/g at 2℃ and 10℃ for 60 and 10 days, respectively. The growth of L. monocytogenes at the bottom of mushrooms was faster than that at the top at both the temperatures. These results indicate that the combined SAEW+UVC-W-LED treatment of plastic wrappers and the UVC-LED treatment of mushrooms can be used as potential hurdle technologies to control the risk of L. monocytogenes in mushrooms prior to packaging at farms.

팽이버섯에서 권지로 L. monocytogenes의 교차오염 가능성과 권지의 세척·소독과정에서 SAEW와 UVC-W-LED 방수 모듈 병합효과를 확인하였다. 또한 팽이버섯의 부위별 L. monocytogenes의 생장과 포장 전 팽이버섯에서 L. monocytogenes를 저감화 할 수 있는 UVC-LED 조사 처리하여 효과를 분석하였다. 오염된 권지를 소독하기 위하여 SAEW와 UVC-W-LED를 병합처리 했을 때 L. monocytogenes의 오염도를 최대 99.9% 이상 저감화시켜 권지의 세척·소독에 활용 가능성을 확인하였다. 또한 오염된 권지의 L. monocytogenes가 팽이버섯에 전이되어 권지 표면을 주기적으로 세척·소독 작업이 필요하다. 팽이버섯의 유통 온도인 2℃에서 60일, 10℃ 저장 시 10일 저장기간 동안 팽이버섯 부위에 상관없이 L. monocytogenes 모두 3 log CFU/g 이상 성장함을 확인하였으며 아랫부분에서 성장이 더 빠르게 일어나는 것을 확인하였다. 팽이버섯에 오염된 L. monocytogenes의 초기 오염도가 2.76 log CFU/g였을 때 UVC-LED 3분 조사 후 0.47 log CFU/g 감소하는 것을 확인하였다. UVC-LED 조사 처리는 초기 오염도에 따라 영향을 받는 것으로 확인되어 실제 팽이버섯에 L. monocytogenes의 초기 오염도를 2 log CFU/g 이하로 낮게 오염시켰을 때 저감화 효과는 0.81 log CFU/g으로 두 배 증가하였다. 그러나 UVC-LED가 표면 살균 효과만 있고 UVC-LED를 조사한 팽이버섯에서도 L. monocytogenes의 증식이 완전 억제되는 것은 아니므로 포장방법 개선 등과 같은 추가 기술 적용이 필요하다. 앞으로, 팽이버섯 재배 농장에서도 안전하고 품질이 우수한 팽이버섯이 국내·외로 공급될 수 있도록 위생관리 강화가 필요하다.

Keywords

Acknowledgement

본 연구는 2020년도 J&K 연구소 연구지원(과제 번호: 20212022)에 의하여 이루어진 것이며, 연구비 지원에 감사드립니다.

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