The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Inhibitory Effects of Aureobasidium pullulans MHAU2101 Isolated from Domestic Pear Blossom Against Fire Blight

국내 배나무 꽃에서 분리한 Aureobasidium pullulans MHAU2101의 화상병 발생 억제 효과

  • Hyeonseok Oh (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Hyo-Won Choi (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Yong Hwan Lee (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Seung Yeup Lee (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Mi-Hyun Lee (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Sang-Keun Oh (Department of Applied Biology, Chungnam National University)
  • 오현석 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 최효원 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이용환 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이승엽 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이미현 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 오상근 (충남대학교 농업생명과학대학 응용생물학과)
  • Received : 2022.11.09
  • Accepted : 2022.12.09
  • Published : 2022.12.31
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Abstract

This study was conducted to identify yeast species isolated from domestic pear blossom through gene sequencing and analysis of morphological characteristics, and to confirm specific yeast species inhibitory effects toward fire blight in immature apples, pears, and crab apple blossoms. Yeast morphological characteristics were consistent with the known characteristics of Aureobasidium pullulans. Nucleotide sequencing of the D1/D2 region of large-subunit (LSU) 26S ribosomal DNA and the internal transcribed spacer (ITS) region confirmed its identity as A. pullulans (MHAU2101). Inoculation of immature fruits with A. pullulans MHAU2101 before exposure to Erwinia amylovora prevented fire blight symptoms in apples and pears. A. pullulans MHAU2101 treated crab apple blossoms had a significantly lower flower infection rate than untreated blossoms, revealing 64% of the potency of streptomycin. The A. pullulans MHAU2101 treated group also displayed lower E. amylovora density in both pistil and hypanthium compared to the untreated group, especially in the hypanthium. This study confirms that A. pullulans MHAU2101 isolated from domestic pear blossom can effectively suppress the onset of fire blight.

본 연구는 국내 배나무 꽃에서 분리한 효모의 형태학적 특성 및 유전자 염기서열 분석을 통해 종을 동정하고, 사과와 배의 미숙과와 꽃사과의 꽃을 대상으로 화상병 발병 억제효과를 확인하고자 수행하였다. 효모의 형태적 특성을 조사한 결과 기존 A. pullulans의 특성과 일치하였으며, large-subunit (LSU) 26S ribosomal DNA의 D1/D2 영역과 internal transcribed spacer (ITS) 영역의 염기서열을 분석한 결과 A. pullulans (MHAU2101)로 동정되었다. E. amylovora를 미숙과에 접종하기 전에A. pullulans MHAU2101을 처리할 경우 사과와 배의 미숙과에서 모두 화상병 병징이 나타나지 않았다. 꽃사과 꽃에서 화상병 방제 효과를 검정한 결과, A. pullulans MHAU2101 처리구는 무처리구에 비해 꽃 감염률이 유의하게 낮았으며, 스트렙토마이신 수화제 대비 64%의 방제효과를 보였다. 또한A. pullulans MHAU2101 처리구는 무처리구와 비교하여 암술과 화통 모두E. amylovora의 밀도가 낮았으며 특히 화통에서 밀도가 낮은 특징이 있었다. 따라서 본 연구 결과를 통해 국내 배나무 꽃에서 분리한A. pullulans MHAU2101이 화상병 발병을 억제하는데 효과가 있음을 확인하였다.

Keywords

Acknowledgement

This study was carried out with the support of Cooperative Research Programs (Project No. PJ01530202) from Rural Development Administration, Republic of Korea.

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