The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Anti-inflammatory activity of Kyungok-go on Lipopolysaccharide-Stimulated BV-2 Microglia Cells

  • Hyun-Suk Song (Department of Pharmacology, School of Korean Medicine, Wonkwang University) ;
  • Ji-Yeong An (Department of Pharmacology, School of Korean Medicine, Wonkwang University) ;
  • Jin-Young Oh (Department of Pharmacology, School of Korean Medicine, Wonkwang University) ;
  • Dong-Uk Kim (Department of Pharmacology, School of Korean Medicine, Wonkwang University) ;
  • Bitna Kweon (Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University) ;
  • Sung-Joo Park (Hanbang Cardio-Renal Syndrome Research Center, Wonkwang University) ;
  • Gi-Sang Bae (Department of Pharmacology, School of Korean Medicine, Wonkwang University)
  • Received : 2022.07.06
  • Accepted : 2022.09.08
  • Published : 2022.12.01
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Abstract

Objectives: Kyungok-go (KOG) is a traditional multi-herbal medicine commonly used for enforcing weakened immunity for long time. Recently, there are several reports that KOG has anti-inflammatory and immuno-stimulatory activities in many experimental models. However, the protective effects of KOG on neuronal inflammation are still undiscovered. Thus, we investigated the neuro-protective activity of KOG on lipopolysaccharide (LPS)-stimulated mouse microglia cells. To find out KOG's anti-neuroinflammatory effects on microglial cells, we examined the production of nitrite using griess assay, and mRNA expressions of inducible nitric oxide synthase (iNOS), cyclooxygenase (COX)-2 and interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α using real time RT-PCR. In addition, to examine the regulating mechanisms of KOG, we investigated the protein expression of mitogen-activated protein kinases (MAPKs) and Iκ-Bα by western blot. KOG inhibited the elevation of nitrite, iNOS and COX-2 on LPS-stimulated BV2 cells. Also, KOG significantly inhibited the pro-inflammatory cytokines such as IL-1β, IL-6, and TNF-α on LPS-stimulated BV2 microglial cells. Moreover, KOG inhibited the activation of c-Jun N-terminal kinase (JNK), P38 and degradation of Iκ-Bα but not the activation of extracellular signal regulated kinase (ERK) on LPS-stimulated BV2 microglial cells. These results showed KOG has the anti-inflammatory effects through the inhibition on nitrite, iNOS, COX-2, IL-1β, IL-6, and TNF-α via the deactivation of JNK, p38 and nuclear factor (NF)-κB on LPS-stimulated BV2 microglial cells. Thereby, KOG could offer the new and promising treatment for neurodegenerative disease related to neuroinflammation.

Keywords

Acknowledgement

This paper was supported by the Wonkwang University in 2021.

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