The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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Anti-inflammatory Effects of Myrrh Ethanol Extract on Particulate Matter-induced Skin Injury

미세먼지로 인한 피부 각질 세포 손상에서 몰약 에탄올 추출물의 항염증 효과

  • Young Hee Jung (Dept. of Third Medicine, Professional Graduate School of Korean Medicine, Wonkwang University) ;
  • Yeun Wha Roh (Dept. of Korean Pathology, College of Korean Medicine, Wonkwang University) ;
  • Myongsoo Chong (Dept. of Preventive Medicine, College of Korean Medicine & Research Center of Traditional Korean Medicine, Wonkwang University)
  • 정영희 (원광대학교 한의학전문대학원 제3의학과) ;
  • 노연화 (원광대학교 한의과대학 병리학교실) ;
  • 정명수 (원광대학교 한의과대학 예방의학교실 & 원광대학교 전통의학연구소)
  • Received : 2022.03.31
  • Accepted : 2022.07.22
  • Published : 2022.09.01
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Abstract

Objectives: Myrrh have been used as a traditional remedy to treat infectious and inflammatory diseases. However, it is largely unknown whether myrrh ethanol extract could exhibit the inhibitory activities against particulate matter (PM)-induced skin injury on human keratinocytes, HaCaT cells. Therefore, this study was aimed to investigate the inhibitory activity of myrrh ethanol extract on PM-induced skin injury in HaCaT cells. Methods: To investigate the inhibitory effects of myrrh ethanol extract in HaCaT cells, the skin injury model of HaCaT cells was established under PM treatment. HaCaT keratinocyte cells were pre-treated with myrrh ethanol extract for 1 h, and then stimulated with PM. Then, the cells were harvested to measure the cell viability, reactive oxygen species (ROS), pro-inflammatory cytokines including interleukin (IL) 1-beta, IL-6, and tumor necrosis factor (TNF)-𝛼, hyaluronidase, collagen, MMPs. In addition, we examined the mitogen activated protein kinases (MAPKs) and inhibitory kappa B alpha (I𝜅-B𝛼) as inhibitory mechanisms of myrrh ethanol extract. Results: The treatment of myrrh ethanol extract inhibited the PM-induced cell death and ROS production in HaCaT cells. In addition, myrrh ethanol extract treatment inhibited the PM-induced elevation of IL-1beta, IL-6, and TNF-𝛼. Also, myrrh ethanol extract treatment inhibited the increase of hyaluronidase, MMP and decrease of collagen. Furthermore, myrrh ethanol extract treatment inhibited the activation of MAPKs and the degradation of I𝜅-B𝛼. Conclusions: Our result suggest that treatment of myrrh ethanol extract could inhibit the PM-induced skin injury via deactivation of MAPKs and nuclear factor (NF)-𝜅B in HaCaT cells. This study could suggest that myrrh ethanol extract could be a beneficial agent to prevent skin damage or inflammation.

Keywords

References

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