Mycobiology

The Korean Society of Mycology (한국균학회)
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A New Lichen-Forming Fungus, Aspicilia humida, from a Forested Wetland in South Korea, with a Taxonomic Key for Aspicilioid Species of Korea

  • Received : 2021.07.01
  • Accepted : 2021.12.17
  • Published : 2022.02.28
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Abstract

Aspicilia humida Lee is described as a new lichen-forming fungus from a wetland forest, South Korea. The new species is distinguishable from Aspicilia aquatica (Fr.) Körb., the most similar species, by the absence of prothallus, black disk without green color in water, olivebrown epihymenium, shorter hymenium, hymenium I + yellowish blue-green, wider paraphysial tips without a vivid pigment, smaller asci, smaller ascospores, and the presence of stictic acid. Molecular analyses employing internal transcribed spacer (ITS) and mitochondrial small subunit (mtSSU) sequences strongly support A. humida as a distinct species in the A. cinerea group. A surrogate key is provided to assist in the identification of all 28 aspicilioid species of Korea.

Keywords

Acknowledgement

This work was supported by a grant from the Korean National Research Resource Center Program (NRF-2017M3A9B8069471) and the Korean Forest Service Program through the Korea National Arboretum (KNA-202003127AF-00) for the forested wetland conservation of Korea.

References

  1. Magnusson AH. Studies in species of Lecanora, mainly the Aspicilia gibbosa group. Kungl Svenska Vetenskapsakad Arsbok. 1939;3(17):1-182.
  2. Lumbsch HT, Schmitt I, Lucking R, et al. The phylogenetic placement of Ostropales within Lecanoromycetes (Ascomycota) revisited. Mycol Res. 2007;111(3):257-267. https://doi.org/10.1016/j.mycres.2007.01.006
  3. Nordin A, Savic S, Tibell L. Phylogeny and taxonomy of Aspicilia and Megasporaceae. Mycologia. 2010;102(6):1339-1349. https://doi.org/10.3852/09-266
  4. Sohrabi M, Owe-Larsson B, Nordin A, et al. Aspicilia tibetica, a new terricolous species of the himalayas and adjacent regions. Mycol Prog. 2010;9(4):491-499. https://doi.org/10.1007/s11557-010-0656-7
  5. Haji Moniri M, Gromakova AB, Lokos L, et al. New members of the Megasporaceae (Pertusariales, lichen-forming Ascomycota): Megaspora iranica spec. nova and Oxneriaria gen. nova. Acta Bot Hung. 2017;59(3-4):343-370. https://doi.org/10.1556/034.59.2017.3-4.5
  6. Wheeler TB. Multilocus phylogeny of the lichen family Megasporaceae [MS thesis]. Missoula, MT: University of Montana; 2017.
  7. Clauzade G. Les genres Aspicilia Massal. et Bellemerea Hafellner et Roux. Bull Soc Bot Cent.-Ouest. 1984;15:127-141.
  8. Wirth V. Die flechten Baden-Wurttembergs: Verbreitungsatlas. Stuttgart: E. Ulmer; 1987.
  9. Hafellner J. Die Gattung Aspicilia, ihre Ableitungen nebst Bemerkungen uber cryptolecanorine Ascocarporganisation bei anderen Genera der Lecanorales (Ascomycetes lichenisati). ABM. 1991;16:133-140. https://doi.org/10.24310/abm.v16i.9153
  10. Sohrabi M, Leavitt SD, Halici MG, et al. Teuvoa, a new lichen genus in Megasporaceae (Ascomycota: Pertusariales), including Teuvoa junipericola sp. nov. Lichenologist. 2013;45(3):347-360. https://doi.org/10.1017/s0024282913000108
  11. Zakeri Z, Divakar PK, Otte V. Taxonomy and phylogeny of Aspiciliella, a resurrected genus of Megasporaceae, including the new species A. portosantana. Herzogia. 2017;30(1):166-176. https://doi.org/10.13158/heia.30.1.2017.166
  12. Lucking R, Hodkinson BP, Leavitt SD. The 2016 classification of lichenized fungi in the Ascomycota and Basidiomycota-approaching one thousand genera. Bryologist. 2017;119(4):361-416. https://doi.org/10.1639/0007-2745-119.4.361
  13. Hue A. Lichenes morphologice et anatomice. Genus 48 - Aspicilia. [ser. 5.]. Nouv Arch Mus Hist Nat. 1910;2:1-120.
  14. Kondratyuk S, Lokos L, Tschabanenko S, et al. New and noteworthy lichen-forming and lichenicolous fungi. Acta Bot Hung. 2013;55(3-4):275-349. https://doi.org/10.1556/ABot.55.2013.3-4.9
  15. Aptroot A, Moon KH. 114 New reports of microlichens from Korea, including the description of five new species, show that the microlichen flora is predominantly Eurasian. Herzogia. 2014;27(2):347-365. https://doi.org/10.13158/heia.27.2.2014.347
  16. Kondratyuk SY, Lokos L, Park JS, et al. New Aspicilia species from South Korea proved by molecular phylogeny with a key to the Eastern Asian aspicilioid lichens. Studia Bot Hung. 2016;47(2):227-249.
  17. Kondratyuk SY, Lokos L, Halda JP, et al. New and noteworthy lichen-forming and lichenicolous fungi 5. Acta Bot Hung. 2016;58(3-4):319-396. https://doi.org/10.1556/ABot.58.2016.3-4.7
  18. Paukov A, Nordin A, Roux C, et al. Lectotypification and synonymization of some Aspicilia species (Megasporaceae, Ascomycota) described by A. Hue from Korea and Japan. Phytotaxa. 2017;291(1):94-98. https://doi.org/10.11646/phytotaxa.291.1.10
  19. Kondratyuk SY, Lokos L, Halda JP, et al. New and noteworthy lichen-forming and lichenicolous fungi 6. Acta Bot Hung. 2017;59(1-2):137-260. https://doi.org/10.1556/034.59.2017.1-2.7
  20. Yakovchenko L, Davydov EA, Paukov A, et al. New lichen records from Korea-I. Mostly arctic-alpine and tropical species. Herzogia. 2018;31(2):965-981. https://doi.org/10.13158/heia.31.2.2018.965
  21. Orange A, James PW, White FJ. Microchemical methods for the identification of lichens. London: British Lichen Society; 2001.
  22. White TJ, Bruns T, Lee S, et al. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: a Guide to Methods and Applications. 1990;18(1):315-322.
  23. Zoller S, Scheidegger C, Sperisen C. PCR primers for the amplification of mitochondrial small subunit ribosomal DNA of lichen-forming ascomycetes. Lichenologist. 1999;31(5):511-516. https://doi.org/10.1006/lich.1999.0220
  24. Rehner SA, Samuels GJ. Taxonomy and phylogeny of Gliocladium analysed from nuclear large subunit ribosomal DNA sequences. Mycol Res. 1994;98(6):625-634. https://doi.org/10.1016/s0953-7562(09)80409-7
  25. Ekman S. Molecular phylogeny of the Bacidiaceae (Lecanorales, lichenized Ascomycota). Mycol Res. 2001;105(7):783-797. https://doi.org/10.1017/S0953756201004269
  26. Hall TA. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser. 1999;41:95-98.
  27. Stecher G, Tamura K, Kumar S. Molecular evolutionary genetics analysis (MEGA) for macOS. Mol Biol Evol. 2020;37(4):1237-1239. https://doi.org/10.1093/molbev/msz312
  28. Edler D, Klein J, Antonelli A, et al. raxmlGUI 2.0 beta: a graphical interface and toolkit for phylogenetic analyses using RAxML. Methods Ecol Evol. 2021;12(2):373-377. https://doi.org/10.1111/2041-210X.13512
  29. Bouckaert R, Vaughan TG, Barido-Sottani J, et al. BEAST 2.5: an advanced software platform for bayesian evolutionary analysis. PLoS Comput Biol. 2019;15(4):e1006650 https://doi.org/10.1371/journal.pcbi.1006650
  30. Bouckaert RR, Drummond AJ. bModelTest: Bayesian phylogenetic site model averaging and model comparison. BMC Evol Biol. 2017;17(1):42. https://doi.org/10.1186/s12862-017-0890-6
  31. Rambaut A. FigTree v1.4.2 [Internet]. Edinburgh: University of Edinburgh; 2014. [cited 2020 Jul 4]. Available from: http://tree.bio.ed.ac.uk/software/figtree.
  32. Lee BG, Hur JS. A new lichenized fungus, Lecanora baekdudaeganensis, from South Korea, with a taxonomic key for korean Lecanora species. MycoKeys. 2020;70:39-58. https://doi.org/10.3897/mycokeys.70.51569
  33. Nash TH, III Gries C, Bungartz F. Lichen flora of the greater sonoran desert region., Vol. III. Tempe, AZ, U.S.A.: Lichens Unlimited/Arizona State University; 2007.
  34. Smith CW, Aptroot A, Coppins BJ, et al. The lichens of great britain and Ireland. London, UK: The British Lichen Society; 2009.
  35. Roux C, Bertrand M, Nordin A. Aspicilia serenensis Cl. Roux et M. Bertrand sp. nov., espece nouvelle de lichen (groupe d'A. calcarea, Megasporaceae. Bull Soc Linn Provence. 2016;67:165-182.