Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Zinc-Solubilizing Streptomyces spp. as Bioinoculants for Promoting the Growth of Soybean (Glycine max (L.) Merrill)

  • Chanwit Suriyachadkun (Thailand Bioresource Research Center, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)) ;
  • Orawan Chunhachart (Division of Microbiology, Department of Science, Faculty of Liberal Arts and Science, Kasetsart University Kamphaeng Saen Campus) ;
  • Moltira Srithaworn (Division of Microbiology, Department of Science, Faculty of Liberal Arts and Science, Kasetsart University Kamphaeng Saen Campus) ;
  • Rungnapa Tangchitcharoenkhul (Graduate School, Suan Dusit University) ;
  • Janpen Tangjitjareonkun (Department of Resources and Environment, Faculty of Science at Sriracha, Kasetsart University, Sriracha Campus)
  • Received : 2022.06.27
  • Accepted : 2022.10.12
  • Published : 2022.11.28
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Abstract

Zinc-solubilizing bacteria can convert the insoluble form of zinc into soluble forms available to plants. This study was conducted to isolate and screen zinc-solubilizing actinobacteria from rhizosphere soils and to assess their effect on vegetable soybean growth. In total, 200 actinobacteria strains belonging to 10 genera were isolated from rhizosphere soil samples. Among these isolates, four showed zinc solubilization with solubilizing index values ranging from 3.11 to 3.78 on Bunt and Rovira agar supplemented with 0.1% zinc oxide. For the quantitative assay, in broth culture, strains CME34 and EX51 solubilized maximum available zinc contents of 529.71 and 243.58 ㎍/ml. Furthermore, indole-3-acetic acid (IAA) and ammonia were produced by these two strains, the strain CME34 produced the highest amount of IAA 4.62 ㎍/ml and the strain EX51 produced the highest amount of ammonia 361.04 ㎍/ml. In addition, the phosphate-solubilizing abilities in Pikovskaya's medium of CME34 and EX51 were 64.67 and 115.67 ㎍/ml. Based on morphological and biochemical characterization and 16S rDNA sequencing, the strains CME34 and EX51 were closely related to the genus Streptomyces. In a greenhouse experiment, single-strain inoculation of Streptomyces sp. CME34 or EX51 significantly increased the shoot length, root length, plant dry weight, number of pods per plant and number of seeds per plant of vegetable soybean plants compared to the uninoculated control. These findings facilitated the conclusion that the two Streptomyces strains have potential as zinc solubilizers and can be suggested as bioinoculants to promote the growth and yield of soybean.

Keywords

Acknowledgement

This work was supported by the Kasetsart University Research and Development Institute (KURDI), Bangkok, Thailand under grant no. FF(KU)18.64. We thank Prof. Dr. Uthirat Na-Nakorn for her helpful discussion. Mr. Ongart Tara, Ms. Kanyanat Sartprem and Ms. Sasithorn Saetia provided technical assistance.

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