Research in Plant Disease (식물병연구)

The Korean Society of Plant Pathology (한국식물병리학회)
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First Report of Bacterial Wilt by Ralstonia pseudosolanacearum on Peanut in Korea

Ralstonia pseudosolanacearum에 의한 땅콩 풋마름병 발생 보고

  • Choi, Soo Yeon (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Nam Goo (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kim, Sang-Min (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Lee, Bong Choon (Crop Foundation Research Division, National Institute of Crop Science, Rural Development Administration)
  • 최수연 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 김남구 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 김상민 (농촌진흥청 국립식량과학원 작물기초기반과) ;
  • 이봉춘 (농촌진흥청 국립식량과학원 작물기초기반과)
  • Received : 2021.12.14
  • Accepted : 2022.02.11
  • Published : 2022.03.31
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Abstract

A peanut plant showing wilt and browned symptom was found in the field of Gochang, Korea, in July 2021. The symptomatic peanut plant was collected from the field and isolation of the pathogen caused the wilt symptom was performed using the collected sample on TZC media. The dominated colony on media was isolated colony on media was isolated and subcultured of purification. The pure cultured bacteria was identified as Ralstonia solanacearum by sequencing of 16S rRNA gene. Multiplex polymerase chain reaction using phylotype-specific primer set identified isolate as phylotype I (R. pseudosolanacearum). Phylogenetic tree was constructed based on 16S rRNA sequence and it was closed with R. pseudosolanacearum. Pathogenicity of the isolates was assessed by soil drenching inoculation on 4-week-old peanut plant. The wilt symptom was successfully reproduced by inoculation of the isolates after 14 days. This is first report of bacterial wilt caused by R. pseudosolanacearum on peanut in Korea.

2021년 7월, 고창 땅콩 재배포장에서 시들음 증상을 보이는 땅콩 지상부를 발견하였다. 병징은 잎이 갈색으로 시들어 말라 죽은 것처럼 보였으며, 채집한 식물체의 지제부를 잘라 표면소독 후 멸균수에 넣었을 때 ooze 현상을 관찰하였다. 땅콩에서 순수분리된 병원균은 16s rRNA 유전자 염기서열과 phylotype 분류, 유연관계 분석을 통해 분리된 균주가 Ralstonia pseudosolanacearum이라는 것을 확인하였다. 현재까지 국내에 보고된 풋마름병은 고추, 토마토, 감자 등을 기주로 발생한다고 알려져 있다. 본 연구는 국내 처음으로 R. pseudosolanacearum에 의해 발생한 땅콩 풋마름병을 보고하고자 한다.

Keywords

Acknowledgement

This research was carried out with the support of "Cooperative Research Program for Agriculture Science & Technology Development (project No. PJ016095022021)" from Rural Development Administration, Republic of Korea.

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