Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Immunostimulatory Activity of Hibiscus syriacus L. Leaves in Mouse Macrophages, RAW264.7 cells, and Immunosuppressed Mice

  • Na Gyeong, Geum (Department of Medicinal Plant Resources, Andong National University) ;
  • Ju Hyeong, Yu (Department of Medicinal Plant Resources, Andong National University) ;
  • So Jung, Park (Department of Medicinal Plant Resources, Andong National University) ;
  • Min Yeong, Choi (Department of Medicinal Plant Resources, Andong National University) ;
  • Jae Won, Lee (Department of Medicinal Plant Resources, Andong National University) ;
  • Gwang Hun, Park (Forest Medicinal Resources Research Center, National Institute of Forest Science) ;
  • Hae-Yun, Kwon (Special Forest Resources Division, National Institute of Forest Science) ;
  • Jin Boo, Jeong (Department of Medicinal Plant Resources, Andong National University)
  • Received : 2022.05.31
  • Accepted : 2022.06.28
  • Published : 2022.12.01
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Abstract

Under the COVID-19 pandemic, interest in immune enhancement is increasing. Although the immune-enhancing activity of plants of the genus Hibiscus has been reported, there is no study on the immune-enhancing activity of H. syriacus. Thus, in this study, we investigated the immune-enhancing activity of Hibiscus syriacus leaves (HSL) in mouse macrophages, RAW264.7 cells, and immunosuppressed mice. HSL increased the production of immunostimulatory factors such as nitric oxide (NO), inducible nitric oxide synthase (iNOS), interleukin-1β (IL-1β), and tumor necrosis factor-α (TNF-α) and activated the phagocytosis in RAW264.7 cells. The HSL-mediated production of immunostimulatory factors was dependent on toll-like receptor 4 (TLR4), p38, and c-Jun N-terminal kinase (JNK) in RAW264.7 cells. In the immunosuppressed mouse model, HSL increased the spleen index, the levels of the cytokines, and the numbers of lymphocytes, neutrophils, and monocytes. Taken together, HSL may be considered to have immune-enhancing activity and be expected to be used as a potential immune-enhancing agent.

Keywords

Acknowledgement

This work was supported by a grant from National Institute of Forest Science in 2021 (project number: FG0403-2018-01-2021) and R&D Program for Forest Science Technology (Project No. 2021377C10-2123-BD02) provided by Korea Forest Service (Korea Forestry Promotion Institute).

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