Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Modification of Insect Sodium Currents by a Pyrethroid Permethrin and Positive Cooperativity with Scorpion Toxins

피레스로이드계 살충제 퍼메트린이 Heliothis virescens 중추신경세포에 있는 나트륨채널에 작용하는 기작을 전기생리학적으로 연구

  • Lee, Daewoo (Neuroscience Program, Department of Biological Sciences, Ohio University) ;
  • Adams, Michael E. (Depts. of Entomol. and Cell Biol. & Neurosci., Univ. of California)
  • 이대우 (오하이오 대학교, 생물학과, 신경과학프로그램) ;
  • 마이클 아담스 (캘리포니아 주립대학교, 곤충 및 세포신경생물학과)
  • Received : 2021.12.30
  • Accepted : 2022.02.22
  • Published : 2022.03.01
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Abstract

In this study, we have examined pyrethroid actions on sodium channels in the pest insect Heliothis virescens. The synthetic pyrethroid permethrin increased steady-state sodium current in H. virescens central neurons and prolonged tail currents (INa-tail) due to extreme slowing of sodium channel deactivation. Prolongation of INa-tail was evident at permethrin concentrations as low as 60 nM, which modified ~1.7% of sodium channels and 10 μM permethrin modified about 30% of channels. The average time constant (τ1) of tail current decay was ~335 ms for permethrin-modified channels. These modified channels activated at more negative potentials and showed slower activation kinetics, and failed to inactivate. Permethrin modification of sodium channels was dramatically potentiated by the α scorpion toxin LqhαIT, showing positive cooperativity between two binding sites. The amplitude of the tail current induced by 0.3 μM permethrin was enhanced ~8-fold by LqhαIT (200 pM). Positive cooperativity was also observed between permethrin and the insect-specific scorpion toxin AaIT as 10 nM permethrin potentiated the shift of voltage dependence caused by AaIT (~2-fold).

본 연구는 피레스로이드계 살충제인 퍼메트린이 Heliothis virescens의 중추신경세포의 나트륨채널에 어떻게 작용하는 가를 전기생리학적으로 관찰하였다. 퍼메트린은 나트륨채널의 꼬리전류(INa-tail)를 지속적으로 증가시켰으며 이러한 비정상적인 나트륨 전류증가가 나방류의 신경계에 과도한 흥분을 일으겨 살충작용을 하는 것으로 생각된다. 이러한 살충작용은 전갈독과 함께 사용했을때 약 8배의 증가가 있었음을 확인하였다. 전갈독이 살충제의 독성을 강화하는 분자생리학적 기전연구가 계속되면 해충방제에 많은 기여를 할 것으로 생각된다.

Keywords

Acknowledgement

저의 학문적 스승이셨던 고 부경생 교수님의 추모논문집에 이 논문을 발표하게 됨을 개인적으로 매우 의미있게 생각합니다. 논문을 준비하면서 참 스승이셨던 교수님을 다시한번 생각할 수 있는 시간이었음을 감사드립니다. University of California-Riverside박사과정 대학원생으로 이논문실험을 하던 1995년 여름에 교수님과 사모님이 Riverside를 약1주일가량 방문하셨을 때 , 이 실험결과와 곤충생리학 전반에 대해 귀한 조언을 해주시고 지도해 주시던 일들이 생각납니다. 박사과정 후에는 다른 분야를 연구하게 되어 이연구결과를 논문으로 투고하기가 쉽지 않았는데, 25년이 넘어 교수님으로 인해 출판하게 되니 교수님의 생전동안의 제자사랑과 또 고인이 되신 후에도 스승님의 사랑을 알게 하시니 참 감사하기도 하고 또 많이 그립고 아쉽습니다. 다시 한 번 교수님의 명복을 빕니다.

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