Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Properties of β-carotene-loaded chitosan/hyaluronic acid nanocapsules: solubility and redispersibility

베타카로틴 함유 키토산/하이알루론산 나노캡슐의 용해도 및 재분산성 특성

  • An, Eun Jung (Department of Food and Nutrition, Hanyang University) ;
  • Lee, Ji-Soo (Department of Food and Nutrition, Hanyang University) ;
  • Lee, Hyeon Gyu (Department of Food and Nutrition, Hanyang University)
  • 안은정 (한양대학교 식품영양학과) ;
  • 이지수 (한양대학교 식품영양학과) ;
  • 이현규 (한양대학교 식품영양학과)
  • Received : 2021.11.04
  • Accepted : 2021.12.31
  • Published : 2022.02.28
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Abstract

To improve the solubility of β-carotene, three types of β-carotene-loaded nanocapsules were prepared using chitosan (CS) and two cross-linkers, sodium tripolyphosphate (TPP) and hyaluronic acid (HA), alone or in combination (CS-TPP, CS-TPP-HA, and CS-HA). The entrapment efficiency of all nanocapsules significantly increased with an increase in TPP and HA, with the efficiency ranging from 95% to 99%. The solubility of β-carotene was significantly improved by CS nanoencapsulation before and after lyophilization and during storage. CS/HA nanoencapsulation significantly improved (by 11-fold) the water solubility of β-carotene. In particular, CS/HA nanoencapsulation was the most effective in terms of not only the solubility of β-carotene, but also the redispersibility ratio. Therefore, CS/HA encapsulation could be useful for improving the solubility of poorly soluble active ingredients, such as β-carotene.

β-Carotene의 용해도를 증진시키기 키토산을 기본 피복물질로 사용하면서 TPP와 HA를 단독 또는 복합적으로 사용하여 세 종류(CS/TPP, CS/TPP/HA와 CS/HA)의 나노캡슐을 제조하였다. 모든 종류의 나노캡슐에서 95%가 넘는 높은 포집효율이 관측되었다. β-Carotene 에멀젼의 저장안정성이 취약한 pH 2-3 조건에서 8일동안 저장하였을 때 CS/TPP/HA와 CS/HA 나노캡슐의 물리적 특성뿐만 아니라 β-carotene의 용해도 또한 안정적으로 유지되는 경향을 나타냈다. β-Carotene의 용해도를 나노캡슐의 건조 전과 후 관측한 결과, 대조군에 비해서 나노캡슐화에 의해서 유의적으로 향상된 것을 확인할 수 있었다. 특히 CS/HA 나노캡슐은 용해도와 재분산성의 측면에서 모두 우수한 특성을 나타냈다. 본 연구를 통해서 CS/HA 나노캡슐은 β-carotene과 같은 난용성 활성성분의 용해도를 증진시킬 수 있는 식품산업에 활용가능한 전달체로서 이용 가능성이 높을 것으로 판단된다.

Keywords

Acknowledgement

이 논문은 2021년도 정부(과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행된 연구임(2021R1A2C2013460).

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