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뽕나무발효톱밥을 이용한 흰점박이꽃무지(딱정벌레목: 꽃무지과) 유충 사육에서 적정 사육밀도 및 저온처리 조건

Optimal Larval Density and Low Temperature Storage Conditions for Rearing of Protaetia brevitarsis (Coleoptera: Cetoniidae) using a Fermented Mulberry Sawdust-base Diet

  • 임주락 (전라북도농업기술원 잠사곤충시험장) ;
  • 문형철 (전라북도농업기술원 잠사곤충시험장) ;
  • 박나영 (전라북도농업기술원 잠사곤충시험장) ;
  • 이상식 (전라북도농업기술원 잠사곤충시험장) ;
  • 김웅 (전라북도농업기술원 잠사곤충시험장) ;
  • 최창학 (전라북도농업기술원 잠사곤충시험장) ;
  • 김희준 (전라북도농업기술원 잠사곤충시험장)
  • Ju-Rak, Lim (Sericulture and Entomology Experiment Station, Jeollabuk-do Agricultural Research and Extension Services) ;
  • Hyung-Cheol, Moon (Sericulture and Entomology Experiment Station, Jeollabuk-do Agricultural Research and Extension Services) ;
  • Na-Young, Park (Sericulture and Entomology Experiment Station, Jeollabuk-do Agricultural Research and Extension Services) ;
  • Sang-Sik, Lee (Sericulture and Entomology Experiment Station, Jeollabuk-do Agricultural Research and Extension Services) ;
  • Woong, Kim (Sericulture and Entomology Experiment Station, Jeollabuk-do Agricultural Research and Extension Services) ;
  • Chang-Hak, Choi (Sericulture and Entomology Experiment Station, Jeollabuk-do Agricultural Research and Extension Services) ;
  • Hee-Jun, Kim (Sericulture and Entomology Experiment Station, Jeollabuk-do Agricultural Research and Extension Services)
  • 투고 : 2022.04.11
  • 심사 : 2022.10.18
  • 발행 : 2022.12.01

초록

흰점박이꽃무지(Protaetia brevitarsis seulensis) 유충을 사육상자(가로 543 × 세로 363 × 높이 188 mm) 안에서 뽕나무 발효톱밥 기반 사료를 이용하여 집단으로 사육할 때(25℃, 16:8 L:D), 투입될 수 있는 갓 부화한 유충의 밀도를 검토하였다. 100-175마리 투입구는 90일후 생존율이 80% 이상으로 사육밀도 사이에 차이가 없었다. 그러나 200마리 밀도에서는 생존율이 약 10% 이상 감소하였다. 100-150마리 밀도에서 유충 평균 무게는 처리 밀도 사이에 유의한 차이가 없었으나, 175마리와 200마리 밀도에서는 100마리 밀도에 비해 유충 무게가 유의하게 낮았다. 사육밀도가 낮을수록 유충 무게가 더 빨리 증가하였다. 이상의 결과를 종합하여 100-150마리가 처음 투입밀도로 적절하다고 판단하였다. 두 번째 실험으로, 종령 유충을 저온에 보관하는 조건을 검토하였다. 종령 유충을 4개의 무게 집단(1.8-2.0, 2.0-2.3, 2.3-2.5, 2.5 g 이상)으로 구분하여 4, 8, 10℃에 보관하였을 때, 4℃에서는 70일 보관된 유충들은 무게에 관계없이 모두 사망하였다. 그러나 8℃에서는 70일 보관집단에서 약 80%의 유충이 생존하였다. 보관하는 유충의 무게가 무거울수록 생존율이 높았다. 4℃ 보관에서는 무게가 2.3 g 이상인 유충을 50일까지 보관한 후 우화한 성충들만 적은 수의 산란을 하였다. 8℃와 10℃ 처리에서는 유충 무게에 관계 없이 모두 산란을 하였다. 이상의 결과로부터 무게가 2.3 g 이상인 흰점박이꽃무지 종령 유충을 8℃에서 30-50일 범위에서 저장할 수 있다고 판단하였다.

We raised the white-spotted flower chafer, Protaetia brevitarsis seulensis, from the larvae stage using a fermented mulberry sawdust-base diet at 25℃ and 16:8 h light:dark photoperiod. First, we determined the optimal density of the neonate larvae to be introduced into a rearing box (543 × 363 × 188 mm). The survival rates of the larvae were higher than 80% at 90 days after rearing at 100-175 larval densities but reduced by more than 10% at 200 larval density. The larval weights at 100 to 150 larval densities were similar; however, the weights at 175 and 200 larval densities were lower than those at 100 larval densities, indicating that the rate of weight gain increased under lower density. Based on these results, we inferred that 100-150 larvae was the optimal density. Second, we investigated the storage conditions of the last instar larvae under low temperatures. Four weight groups (1.8-2.0, 2.0-2.3, 2.3-2.5, and 2.5 g) of larvae were stored at 4, 8, and 10℃, respectively. All the larvae at 4℃ died 70 days after storage, whereas 80% of the larvae survived at 8℃ 70 days after storage, indicating that high larval weight was associated with high survival rates. The adults that emerged from larvae with more than 2.3 g and stored for up to 50 days at 4℃ laid few eggs. However, the adults that emerged from all larval weight groups stored for 70 days at 8℃ and 10℃ laid several eggs successfully. Based on these results, we inferred that the last instar larvae with more than 2.3 g could be stored for 30-50 days at 8℃.

키워드

과제정보

본 연구는 농촌진흥청 지역특화작목기술개발과제(과제번호 : PJ012574)의 지원에 의하여 연구가 수행되었습니다.

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