Journal of Korean Neurosurgical Society

The Korean Neurosurgical Society (대한신경외과학회)
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The Effect of Genetically Modified Lactobacillus plantarum Carrying Bone Morphogenetic Protein 2 Gene on an Ovariectomized Rat

  • Jin, Eun-Sun (Department of Internal Medicine, College of Medicine, Kyung Hee University) ;
  • Kim, Ji Yeon (Laboratory of Stem Cell Therapy, Asan Medical Center, College of Medicine, University of Ulsan) ;
  • Yang, Jung-Mo (Department of Biotechnology, Korea National University of Transportation) ;
  • Kim, Jun-Sub (Department of Biotechnology, Korea National University of Transportation) ;
  • Min, JoongKee (Laboratory of Stem Cell Therapy, Asan Medical Center, College of Medicine, University of Ulsan) ;
  • Jeon, Sang Ryong (Laboratory of Stem Cell Therapy, Asan Medical Center, College of Medicine, University of Ulsan) ;
  • Choi, Kyoung Hyo (Laboratory of Stem Cell Therapy, Asan Medical Center, College of Medicine, University of Ulsan) ;
  • Moon, Gi-Seong (Department of Biotechnology, Korea National University of Transportation) ;
  • Jeong, Je Hoon (Laboratory of Stem Cell Therapy, Asan Medical Center, College of Medicine, University of Ulsan)
  • Received : 2021.04.19
  • Accepted : 2021.08.04
  • Published : 2022.03.01
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Abstract

Objective : Osteoporosis result from age-related decline in the number of osteoblast progenitors in the bone marrow. Probiotics have beneficial effects on the host, when administered in appropriate amounts. This study investigated the effects of probiotics expressing specific genes, especially the effects of genetically modified bone morphogenetic protein (BMP)-2-expressing Lactobacillus plantarum CJNU 3003 (LP) on ovariectomized rats. Methods : Twenty-eight female Wistar rats (250-300 g, 12 weeks old) were divided into four groups : the sham (control), the ovariectomy (OVX)-induced osteoporosis group (OVX), the OVX and LP (OVX/LP), OVX and genetically modified BMP-2-expressing LP (OVX/LP with BMP) groups. The three groups underwent bilateral OVX and two of these groups were administered two different types of LP via oral gavage daily. At 16 weeks post-OVX, blood was collected from the heart and the bilateral tibiae were extracted and were scanned by ex-vivo micro-computed tomography and stained with hematoxylin-and-eosin (H&E) and Masson's trichrome stain for pathological assessment. The serum levels of osteocalcin (OC), rat C-telopeptide of type I collagen (CTX-I), BMP-2, and receptor activator of nuclear factor-ĸB ligand (RANKL) were measured. Results : The 3D-micro-computed tomography images showed that the trabecular structure in the OVX/LP with BMP group was maintained compared with OVX and OVX/LP groups. No significant differences were detected in trabecular thickness (Tb.Th) between control and OVX/LP with BMP groups (p>0.05). Furthermore, a tendency toward increased BMD, trabecular bone volume, Tb.Th, and trabecular number and decreased trabecular separation was found in rats in the OVX/LP with BMP groups when compared with the OVX and OVX/LP groups (p>0.05). The H&E and Masson's trichrome stained sections showed a thicker trabecular bone in the OVX/LP with BMP group compared with the OVX and OVX/LP groups. There was no difference in serum levels of OC, CTX and RANKL control and OVX/LP with BMP groups (p>0.05). In contrast, significant differences were found in OC and CTX-1 levels between the OVX and OVX/LP with BMP groups (p<0.05). Conclusion : Our results showed that the expression of genetically modified BMP-2 showed inhibition effect for bone loss in a rat model of osteoporosis.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (grant no. NRF-2017R1A2B1005327), and this work was supported by the Soonchunhyang University Research Fund. This document has been checked by at least two professional editors, both of whomare native English speakers.

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