Journal of the Korean Electrochemical Society (전기화학회지)

The Korean Electrochemical Society (한국전기화학회)
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Daily Amperometric Monitoring of Immunoglobulin E in a Mouse Whole Blood: Model of Ovalbumin Induced Asthma

  • Lee, Ju Kyung (Office of Hustar Medical industry innovation university project, Kumoh National Institute of Technology) ;
  • Yoon, Sung-hoon (Inhalation toxicology center for airborne risk factor, Korea institute of toxicology) ;
  • Kim, Sang Hee (Department of Medical IT Convergence, Kumoh National Institute of Technology)
  • Received : 2021.09.23
  • Accepted : 2021.11.03
  • Published : 2022.02.28
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Abstract

There is an increasing interest in monitoring of specific biomarker for determining progression of a disease or efficacy of a treatment. Conventional method for quantification of specific biomarkers as enzyme linked immunosorbent assay (ELISA) has high material costs, long incubation periods, requires large volume of samples and involves special instruments, which necessitates clinical samples to be sent to a lab. This paper reports on the development of an electrochemical biosensor to measure total immunoglobulin E (IgE), a marker of asthma disease that varies with age, gender, and disease in concentrations from 0.3-1000 ng/mL with consuming 20 µL volume of whole blood sample. The sensor provides rapid, accurate, easy, point-of-care measurement of IgE, also, sequential monitoring of total IgE with ovalbumin (OVA) induced mice is another application of sensor. Taken together, these results provide an alternative way for detection of biomarkers in whole blood with low volumes and long-term ex-vivo assessments for understanding the progression of a disease.

Keywords

Acknowledgement

This research was supported by Kumoh National Institute of Technology (2018-104-041)

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