Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Improvement in microbial stability of photosynthetic bacteria via optimized cell immobilization and lyophilization: Application to the treatment of shrimp aquaculture water

  • Received : 2022.11.18
  • Accepted : 2022.12.21
  • Published : 2022.12.30
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Abstract

Photosynthetic bacteria (PSB) play an important role in water purification, and their application is beneficial for sustainable aquaculture. However, maintaining the microbial stability of PSB from subculturing to preservation is a challenging task. Since improvement in the microbial stability of PSB is a crucial parameter, optimized conditions for cell immobilization and lyophilization were investigated. In PSB immobilization, 0.1-M CaCl2 was found to be the most effective divalent metal ion solution in terms of cost-effectiveness, resulting in beads with a 4-mm diameter and high loading (1.91×109 CFU/mL) of viable cells. Maintenance of cell viability, external appearance, and color of PSB beads was best in 3.5% NaCl during storage. In lyophilization, the addition of skim milk (9%) and dextrose (2%) as cryoprotective additives allowed the highest cell viability. Over an 18-week shrimp breeding period, when optimally manufactured beads and lyophilized powder of PSB were applied to shrimp aquaculture water, NH4+, NO3-, and NO2- were more effectively removed by 55%, 100%, and 100%, respectively, compared to controls. Thus, microbial stability of PSB through optimized cell immobilization and lyophilization was successfully enhanced, enabling a wide application.

Keywords

Acknowledgement

We are grateful to Ecobiznet Company (Chuncheon, Gangwon-do, Korea) for invaluable assistance in providing photosynthetic bacterial species, culture media and reactors for the present study.

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