Journal of Veterinary Science

The Korean Society of Veterinary Science (대한수의학회)
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Comparative in vitro biotransformation of fipronil in domestic poultry using liver microsome

  • Kraisiri, Khidkhan (Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University) ;
  • Saranya, Poapolathep (Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University) ;
  • Sittinee, Kulprasertsri (Department of Farm Resources and Production Medicine, Faculty of Veterinary Medicine, Kasetsart University, Kamphaeng Saen Campus) ;
  • Rattapong, Sukkheewan (Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University) ;
  • Paphatsara, Khunlert (Agricultural Toxic Substance Research Group, Agricultural Production Sciences Research and Development Division, Department of Agriculture, Ministry of Agriculture and Cooperatives) ;
  • Mario, Giorgi (Department of Veterinary Sciences, University of Pisa, Via Livornese (latomonte)) ;
  • Amnart, Poapolathep (Department of Pharmacology, Faculty of Veterinary Medicine, Kasetsart University)
  • Received : 2022.06.29
  • Accepted : 2022.09.01
  • Published : 2022.11.30
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Abstract

Domestic poultry are among the non-target species of exposure to fipronil, but limited information is available on the metabolic effects of fipronil exposure in avian. We investigated the comparative capacity of in vitro biotransformation of fipronil among chicken, duck, quail, goose, and rat. Interspecies differences in kinetic parameters were observed; the clearance rate calculations (Vmax/Km) indicated that chicken and duck are more efficient in the cytochrome P450-mediated metabolism of fipronil to sulfone than quail, goose and rat. The lower hepatic clearance of fipronil in quail, goose and rat, suggested that fipronil sulfone may serve as a biomarker to indicate fipronil exposure in these species.

Keywords

Acknowledgement

The authors all thank the financial support of the Smart Start up Research Fund: SRF from the Faculty of Veterinary Medicine, Kasetsart University, Bangkok, Thailand.

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